For modification at the 3' end or internal, it is highly recommended to use purified oligos.
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Quality
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• fully deprotected and desalted, purified by
PAGE or RP-HPLC on request**
• RPC purification available only for CY5;
CY5.5; CY3 labeled oligos and Beckman
Dyes.
• purification is highly recommended for 3' end
and post-synthesis labeling
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Form
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• in concentrated solution, quantitated
• quantity (OD and nmoles) and concentration
(µM and µg/µl)
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Quality Controls
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See information on our oligos: strict quality controls
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Guarantee
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Guaranteed sequence, quality and quantity
(All quantities indicated are minimum guaranteed quantities delivered)
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Documentation
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All Oligos are delivered with an Oligonucleotide Technical Data Sheet that can also be sent through e-mail
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Additional Services
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• Concentration Adjustment
• Aliquoting
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Synthesis Time
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• 3 days for standard fluorescent oligos
• 4 days for PAGE purified oligos, 5 days for RP-HPLC oligos (6 days for larger quantities)
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Storage
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see information on our oligos: storage recommendations
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**RPC purification allows one to obtain hight quantities of purified oligos and will provide the necessary purity level (85-90%) for most hight resolution applications. This purification is available for oligos up to 60 mers.
RP-HPLC purification provides a very pure product with the highest yields. It is advisable to use RP-HPLC purification for oligos ranging from 35 mers to 60 mers. RP-HPLC allows one to reach a purity of approximately 90-98%. For longer oligos the purity level is greatly decreased.
PAGE purification is recommended for oligos from 35 bases up to 100 bases. Although yields can be very low (1 OD range), the level of purity can reach 98% for short oligos, and average 90% for very long oligos.
To learn more about Sigma-Proligo purification methods please see Oligo Quality on our Technical Information Page.
