We offer a valuable service to the molecular biology laboratory - custom designed and assembled genes. Now you can order whole genes, to your exact specifications, as easily as you can order probes and primers. If your research requires custom genes, there's a convenient way to get the exact sequence you need without investing months in lab time. Just call us. We can help you design your synthetic genes (coding sequences) for a variety of applications, including:

Expression - Codon usage is engineered for optimal expression in bacterial, yeast, and plant systems, etc.

Genetic manipulation for protein structure and function studies - Appropriate restriction sites can be included in the gene sequence, dividing the gene into modules or cassettes for protein domain swapping/insertion/deletion/duplication.  In addition, specific mutations may be introduced within a particular gene module.

Gene therapy - Genes can be designed for therapeutic uses such as antisense RNA expression, ribozyme (catalytic RNA) expression and immunotoxin production.

Chimeric/fusion protein production - Coding region domains from several heterologous genes can be linked together.

DNA hybridization - Genes can be constructed from native sequences and modified for cloning into any vector system.  Since these genes contain native sequences, they may be used in hybridization applications and native expression systems.

For more information or to place an order, contact your local Sigma office.