Mass Oligos™ is our high throughput synthesis service for standard oligos that provides timely delivery, reliability of product, and the affordability to meet your budget demands. This is guaranteed by our innovative proprietary UltraFast Parallel Synthesizers (UFPS 24.1) and our highly automated process. We also offer our Mass Oligos™ service with shipment straight to your lab in 24 hours!
The Mass Oligos™ synthesis technology relies on a number of innovations such as:
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- the use of Universal CPG (patented) at the 3' end which eliminates manual selection of the 3' end base.
- use of a 24 well carousel ensuring true parallel synthesis process
- dramatically improved coupling efficiency and coupling time which allow us to produce 24 oligos in less than one hour with very high yields.
Additionally, Proligo Primers & Probes speeds the post-synthesis process by employing state of the art methods such as centralized controlled automation, bar-coding, liquid handling robots. Therefore, we can offer you a range of unbeatable services like concentration adjustments, custom distribution of oligos (mix preparation...), choice of delivery format.
Mass Oligos™ are delivered with an Oligonucleotide Technical Data Sheet that can also be sent through e-mail.
Specifications of Mass Oligos
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Quality control
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All our Mass Oligos undergo vigorous process monitoring and strict batch control, including OD measurement, concentration calculation and mass spectrometry or PAGE analysis.
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Purification
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Fully deprotected and desalted
Purified by RPC upon request
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Guarenteed yield
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Desalted: 2, 5 or 10 OD (approx. 10, 25 or
50 nmols*)
RPC: 2 or 4 OD (approx. 10 or 20 nmols*)
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Length
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15 to 40 mers
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Bases
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DNA (A, C, G or T), degenerate bases (wobbles)
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Backbone
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Phosphodiester bond
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Format
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Delivered in solution in de-ionized water at a specific concentration, if requested
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Storage
and stability
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Although oligonucleotides are stable in solution at 4°C for up to 2 weeks, Proligo recommends storage should be at -20°C. Repetitive freeze-thaw cycles should be avoided by storing as aliquots. Storing at concentrations above 20 µM is recommended. Oligonucleotides with fluorescent labels should be protected from light. Proligo guarantees its oligonucleotides for six months, stored under the above conditions.
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Shipment
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Shipped by express delivery, in solution, in microtubes, deep well or microtiter plates
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Oligonucleotide
Technical Data
Sheet
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Oligonucleotides are delivered with an Oligonucleotide Technical Data Sheet, which includes oligonucleotide name, sequence, concentration, precise quantity in OD and nmols, Tm, MW, size, extinction coefficient and purification information.
We also provide plate and oligonucleotide identification numbers for Mass Oligos.
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Services available upon request
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• Concentration adjustment
• Mixed plate preparation
• Aliquoting
• Custom plate-tracking
• Custom orders
• Additional services may increase
turn-around time
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No. of oligos
per order
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Multiples of 24 oligonucleotides
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Pricing
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Please contact your local Proligo representative
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Ordering
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Web orders only
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*Estimate 1 OD = 5 nmols = 30 µg, for a 20 mer oligo
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