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Quality
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• fully deprotected and desalted, purified by PAGE or RP-HPLC on request**
• it is highly recommended to use purified RNA for oligos > 30 mers.
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Form
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• RNA and 2' O-Methyl mixed oligos are sent dry
• Quantity (OD and nmoles) and concentration (µM and µg/µl)
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Quality Controls
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See information on our oligos: strict quality controls
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Guarantee
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Guaranteed sequence, quality and quantity
Please see yield table above for guaranteed minimum yields
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Documentation
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All Oligos are delivered with an Oligonucleotide Technical Data Sheet that can also be sent through e-mail
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Additional Services
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• Aliquoting
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Synthesis Time
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• 6 days for standard RNA
• 7 days for standard or PAGE purified mixed oligos
• 10 days for RP-HPLC purified oligos
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Storage
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Although oligonucleotides are stable at 4°C for up to 2 weeks, Sigma recommends storing at -20°C. Repetitive freeze-thaw cycles should be avoided by storing as aliquots. For long-term storage, RNA oligos should be dried.
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**RPC purification allows one to obtain hight quantities of purified oligos and will provide the necessary purity level (85-90%) for most hight resolution applications. This purification is available for oligos up to 60 mers.
RP-HPLC purification provides a very pure product with the highest yields. It is advisable to use RP-HPLC purification for oligos ranging from 35 mers to 60 mers. RP-HPLC allows one to reach a purity of approximately 90-98%. For longer oligos the purity level is greatly decreased.
PAGE purification is recommended for oligos from 35 bases up to 100 bases. Although yields can be very low (1 OD range), the level of purity can reach 98% for short oligos, and average 90% for very long oligos.
LNA (Locked Nucleic Acids) is a registered trademark of Exiqon A/S.
