|
Welcome to the Peptide Library Design & Calculator tool presented by Sigma-Aldrich You may launch the Design Tool and reference these tables at the same time, or view the full instructions for getting started.
The protein sequence of interest should use the following letter designations.
| Amino Acid |
3 letter code |
1 letter code |
| Alanine |
Ala |
A |
| Cysteine |
Cys |
C |
| Aspartic acid |
Asp |
D |
| Glutamic acid |
Glu |
E |
| Phenylalanine |
Phe |
F |
| Glycine |
Gly |
G |
| Histidine |
His |
H |
| Isoleucine |
Ile |
I |
| Lysine |
Lys |
K |
| Leucine |
Leu |
L |
| Methionine |
Met |
M |
| Asparagine |
Asn |
N |
| Proline |
Pro |
P |
| Glutamine |
Gln |
Q |
| Arginine |
Arg |
R |
| Serine |
Ser |
S |
| Threonine |
Thr |
T |
| Valine |
Val |
V |
| Tryptophan |
Trp |
W |
| Tyrosine |
Tyr |
Y |
Appropriate peptide length and amino acid overlap:
Hydropathy values presented are the weighted average based upon known hydropathy values per amino acid divided by the length of the peptide sequence. The values are based on model systems of free energy transfer of acetyl-amino acyl amides (Ac-AA-am) by Fauchere & Pliska and based on the RP-HPLC retention times of peptide model systems at pH 7 by Monera.
| Weighted average |
Peptide hydrophobicity classification |
Solubility |
| 1 to 2.25 |
Very hydrophobic |
Very difficult to dissolve |
| 0.6 to 0.9 |
Moderately hydrophobic |
Difficult to dissolve |
| 0.1 to 0.5 |
Hydrophobic |
Likely soluble |
| -0.6 to 0 |
Hydrophilic |
Soluble |
| -1 to –0.5 |
Very hydrophilic |
Very soluble |
Correlations between Fauchere & Pliska and Monera et al Hydrophobicity Scales are shown below:
|
