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 Custom RNA Oligos

Functional Genomics and RNAi
 
   

Attention: The following specifications apply to the USA and Europe. All other countries, click here.

Product Line:
Quality Desalted
Synthesis Scale 0.05 µmole 0.2 µmole 1.0 µmole
Standard RNA X X X
Mixed RNA/DNA/2-OMe/LNA® X X X
Phosphorothioate linkages (s-oligos) X X X

Guaranteed Yields (7-45 mers):
Synthesis Scale 0.05 µmole 0.2 µmole 1.0 µmole
Desalted 5 OD (150µg) 12 OD (360µg) 40 OD (1200µg)
RP-HPLC 1 OD (30µg) 2.5 OD (75µg) 13 OD (390µg)
PAGE 0.5 OD (15µg) 1 OD (30µg) 5 OD (150µg)

Purification:
Quality Purified
Synthesis Scale 0.05 µmole 0.2 µmole 1.0 µmole
RP-HPLC X X X
PAGE X X X


Quality

• fully deprotected and desalted, purified by PAGE or RP-HPLC on request
• it is highly recommended to use purified RNA for oligos > 30 mers.

Form

• RNA and 2' O-Methyl mixed oligos are sent dry
• Quantity (OD and nmoles) and concentration (µM and µg/µl)

Quality Controls

See information on our oligos: strict quality controls

Guarantee

Guaranteed sequence, quality and quantity
Please see yield table above for guaranteed minimum yields

Documentation

All Oligos are delivered with an Oligonucleotide Technical Data Sheet that can also be sent through e-mail

Additional Services

• Aliquoting

Synthesis Time

• 6 days for standard RNA
• 7 days for standard or PAGE purified mixed oligos
• 10 days for RP-HPLC purified oligos

Storage

Although oligonucleotides are stable at 4°C for up to 2 weeks, Sigma recommends storing at -20°C. Repetitive freeze-thaw cycles should be avoided by storing as aliquots. For long-term storage, RNA oligos should be dried.

LNA (Locked Nucleic Acids) is a registered trademark of Exiqon A/S.