|
Spectral properties of fluorophores
The table below details the most commonly used dyes for the fluorescent probes, together with their excitation and emission data. Please enquire for any other labels.
|
Quenchers
|
Maximum absorbance (nm)
|
Maximum emission (nm)
|
|
BHQ-1 Dark Quencher
|
535
|
None
|
|
BHQ-2 Dark Quencher
|
579
|
None
|
|
DABCYL Dark Quencher
|
453
|
None
|
|
DABCYL-dT Dark Quencher
|
453
|
None
|
|
TAMRA
|
550
|
576
|
|
Labels
|
Maximum absorbance (nm)
|
Maximum emission (nm)
|
|
6-FAM
|
495
|
517
|
|
Cy3
|
550
|
570
|
|
Cy5
|
650
|
667
|
|
Cy5.5
|
675
|
694
|
|
Fluorescein
|
495
|
520
|
|
HEX
|
537
|
553
|
|
JOE
|
520
|
548
|
|
LightCycler Red 610
|
590
|
610
|
|
LightCycler Red 640
|
625
|
640
|
|
LightCycler Red 670
|
650
|
670
|
|
LightCycler Red 705
|
680
|
705
|
|
Oregon Green 488
|
495
|
521
|
|
Oregon Green 500
|
499
|
519
|
|
Oregon Green 514
|
506
|
526
|
|
Rhodamine
|
564
|
603
|
|
6-CarboxyRhodamine 6G
|
524
|
557
|
|
Rhodamine Green
|
504
|
532
|
|
Rhodamine Red
|
570
|
590
|
|
ROX
|
581
|
607
|
|
TAMRA
|
550
|
576
|
|
TET
|
521
|
538
|
|
Texas Red
|
589
|
610
|
|
