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Attention: Over the next few weeks, we will be updating our online order system and streamlining product specifications.
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Speed-up your discovery.
- Superior hybridization characteristics
- Enhanced biostability
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LNA Bibliography
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What is LNA?
LNA is a novel type of nucleic acid analog that contains a
2'-O, 4'-C methylene bridge. This bridge restricts the flexibility of the ribofuranose ring and locks the structure into a rigid C3-endo conformation, conferring enhanced hybridization performance and exceptional biostability.
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LNA® oligos are innovative oligonucleotides that contain Locked Nucleic Acids™ (LNA) to provide superior hybridization characteristics and enhanced biostability compared to conventional DNA oligonucleotides.
LNA-containing oligos are provided as follows:
- Deprotected and desalted
- Purified by PAGE, RPC or RP-HPLC upon request
- Delivered in solution in deionized water or dried down
- Systematic QC by MALDI-TOF mass spectrometry analysis and - depending upon the nature of the oligonucleotide requested - by RP-HPLC or IE-HPLC
- Please contact Sigma-Proligo for expected TATs
LNA oligos are composed of either 100% Locked Nucleic Acid (LNA) bases or a mixture of LNA and DNA bases. However, only oligonucleotides that are between 7 and 15 mers in length can be made up of 100% LNA. Oligos heavy in LNA content may exhibit problematic self-complementarity. Therefore, for oligonucleotides longer than 15 mers, we recommend decreasing the LNA content as the oligonucleotide increases in length, as follows:
Length
of LNA oligo |
7 - 15 mers
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16 - 25 mers
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26 - 40 mers
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41 - 60 mers
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Percentage
of LNA |
up to 100%
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up to 60%
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up to 40%
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up to 20%
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Please contact Sigma-Proligo design specialists to discuss application-specific LNA design.
Guaranteed Yields, Labels, Modifications and Turn-Around Time for LNA oligos
LNA oligonucleotides can be designed to enhance a wide variety of genomic applications and technologies that rely upon the use of oligonucleotides:
- Hybridization probes for the detection of nucleic acids and genotyping
- Padlock probes, modified Taqman™ probes and probes for in situ hybridization
- Capture probes for target enrichment, sample preparation and affinity chromatography
- Allele-specific PCR1
- Antisense oligonucleotide technology
- Oligonucleotides for therapeutic or diagnostic applications, such as, aptamers, ribozymes, decoy oligonucleotides and siRNA
- PCR-based applications
In most respects, LNA oligonucleotides can be used in the same manner as DNA oligonucleotides: it is soluble in aqueous buffers, can be ethanol precipitated, dried and resuspended, and analyzed by gel electrophoresis, HPLC and MALDI-TOF.
Specifications of LNA Oligos
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Quality control
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All of our oligonulceotides undergo vigorous process monitoring and strict quality control. Length and labeling are systematically controlled by PAGE or mass spectrometry analysis and - depending on the nature of the oligonucleotide requested, by RP-HPLC or IE-HPLC. Quantity is determined by UV absorbance at 260 nm.
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Purification
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Fully deprotected and desalted
Purified by PAGE, RPC or RP-HPLC upon request
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Length
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7 to 15 mers: up to 100% LNA base content
7 to 60 mers: LNA / DNA mix
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Bases
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LNA (+A, +C, +G or +T) (+ denotes the LNA base)
DNA (A, C, G or T), inosine, degenerate bases (wobbles)
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Backbone
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Phosphodiester and/or phosphorothioate bonds
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Format
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Delivered in solution in deionized water or dried down, upon request
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Storage and stability
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Although oligonucleotides are stable in solution at 4°C for up to 2 weeks, Sigma-Proligo recommends storage should be at -20°C. Repetitive freeze-thaw cycles should be avoided by storing as aliquots. Storing at concentrations above 20 µM is recommended. Oligonucleotides with fluorescent labels should be protected from light. Sigma-Proligo guarantees its oligonucleotides for six months, stored under the above conditions.
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Shipment
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Shipped by express delivery, in individual tubes
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Oligonucleotide
Technical Data
Sheet
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Oligonucleotides are delivered with an Oligonucleotide Technical Data Sheet, which includes oligonucleotide name, sequence, concentration, quantity in OD and nmols, Tm , MW, size, extinction coefficient and purification information.
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Services available upon request
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• Aliquoting
• Adjusted concentration
• Design support
• Additional services may increase turn-around time
• Plate services
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Pricing
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Please contact your local Sigma-Proligo representative
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Ordering
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On-line at www.proligo.com, by email or by fax
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1 PCR (Polymerase Chain Reaction) is patented by Hoffman La Roche Ltd.
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