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Some commonly used quenchers-such as DABCYL and TAMRA-suffer from a number of drawbacks, including poor spectral overlap between the fluorescent dye and quencher molecule (DABCYL) or inherent fluorescence of the quencher (TAMRA)-resulting in a relatively poor signal-to-noise ratio. Black Hole Quencher™ (BHQ™) molecules have been developed to overcome these drawbacks.
Benefits:
- No native fluorescence, resulting in lower background fluorescence
- Increases signal-to-noise ratios, providing higher sensitivity
- Accesses the visible spectrum into near-IR for reporting (480 to 730 nm)
- Maximizes spectral overlap, increasing efficiency of quenching
- Enables wider choice of reporter dyes for multiplexing assays
Absorption Spectra of BHQ dyes among the most commonly used dyes.
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