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LNA® Dual-Labeled Fluorogenic Probes and Molecular Beacons |
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Dual-Labeled DNA Probes |
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| Attention: The following specifications apply to the USA and Europe. All other countries, click here. |
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For your convenience, LNA dual-labeled fluorogenic probes and LNA molecular beacons are available at a fixed price per oligonucleotide, within the following parameters: |
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- Up to 8 LNA bases
- Deprotected, desalted and purified by PAGE or RP-HPLC
- Available in lengths of 7 to 40 mers
- Delivered dried in individual, opaque tubes
- Shipped in 7 working days of receiving your order, pending successful QC validation*
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Guaranteed yields of LNA dual-labeled fluorogenic probes and
LNA molecular beacons
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Guaranteed yield (OD)
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Approximate yield (nmols**)
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Estimated assays (reactions***)
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1
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5
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500
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3
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15
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1,500
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5
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25
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2,500
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10
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50
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10,000
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** Estimate 1 OD = 5 nmols = 30 µg, for a 20 mer oligo
*** Estimated number of assays based on a 50 µl reaction volume.
Please enquire for alternative quantities.
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Labels for LNA dual-labeled fluorogenic probes and
LNA molecular beacons
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5'-end reporter
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3'-end quencher
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6-FAM™, HEX™ or TET™
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TAMRA™, DABCYL, BHQ™-1
or BHQ-2
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Please inquire about optional dye labels.
Figure 1: LNA dual-labeled fluorogenic probes vs. TaqMan® MGB probes in 5' nuclease PCR assays. (Amplification of Staphylococcal enterotoxin gene in a Staphylococcus aureus strain11).
Key
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LNA dual-labeled fluorogenic probe |
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TaqMan MGB probe |
OD = optical density
* Please check with your Sigma-Genosys representative for local delivery times.
Specifications are subject to change.
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qPCR Total Solution Seminar Series
Seminars begin February 7th,
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LNA—Notice of limited rights: Sigma, Sigma-Life Science are only licensed to sell Dual Labeled Fluorescent Probes, LightCycler Probes, Scorpion Probes and Molecular Beacons containing LNA (Locked Nucleic Acids) for use in Real-Time PCR.
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