LNA® LightCycler® Probes

Up to 6 LNA bases
Deprotected, desalted and purified by RP-HPLC
Available in lengths of 7 to 40 mers
Delivered dried in individual, opaque tubes
Shipped in 6 working days of receiving your order, pending successful QC validation*

Guaranteed yields of LNA LightCycler probes
Guaranteed yield (OD)	Approximate yield (nmols)	Estimated assays (reactions)
0.1	0.3	100
0.25	1	300
1.5	3	1,000

Please inquire for alternative quantities.

Labels and modifications for LNA LightCycler probes
	LNA Probe I	LNA Probe 2
	3'-end donor fluorophore	3'-end	5'-end acceptor fluorophore
Labels and modifications	LightCycler Fluorescein	Phosphate	LightCycler Red 640

Note: Fluorescent signaling occurs during the annealing step of Q.PCR.
Please inquire for optional dye labels.

Figure 1: Melting curve profiles for Human Cystic Fibrosis SNP G542X demonstrate the superior discrimination power of LNA LightCycler probes over DNA LightCycler probes.
Figure 1a: A set of DNA LightCycler probes (A-25; D-22)

Key

I	Wild-type template
II	Heterozygous template
III	Mutant-type template
IV	Control without template

Figure 1b: A set of LNA LightCycler probes (A-16; D-16)

Key

I	Wild-type template
II	Heterozygous template
III	Mutant-type template

Note that DNA LightCycler probes do not pick up on the presence of the homozygous mutant, whereas the LNA LightCycler probes are able to identify the presence of all three template types, including the homozygous mutant.

* Please check with your Sigma-Genosys representative for local delivery times.

Specifications are subject to change.

LNA—Notice of limited rights: Sigma, Sigma-Life Science are only licensed to sell Dual Labeled Fluorescent Probes, LightCycler Probes, Scorpion Probes and Molecular Beacons containing LNA (Locked Nucleic Acids) for use in Real-Time PCR.