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Catalog Number N0788
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Product Description
The N-TER™ Nanoparticle siRNA Transfection System is a peptide-based system for use in the transient knockdown of eukaryotic gene expression.1 The N-TER Peptide binds siRNAs non-covalently, forming a nanoparticle.2 This nanoparticle interacts directly with lipids on the surface of the plasma membrane,1,2 allowing the nanoparticle to diffuse across the cell membrane and deliver the siRNA directly to the cytoplasm. Unlike most lipid-based transfection methods, this novel delivery mechanism bypasses the endosomal pathway,1,3,4 eliminating possible compartmentalization and degradation of the siRNA.1,5 |
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Table 1
Cell lines for which the N-TER Nanoparticle siRNA Transfection System have been experimentally validated
| Cell Type |
Source Tissue |
| 3T3-L1, differentiated adipocyte |
mouse embryonic fibroblast cell line |
| A2780 |
human ovarian carcinoma cell line |
| A549 |
human lung carcinoma cell line |
| ASPC-1 |
human pancreatic carcinoma cell line |
| Astrocyte |
human neuronal primary cell |
| Astrocytoma |
human neuronal astrocytoma cell line |
| BSMC |
human bronchial smooth muscle primary cell |
| C2C12, differentiated myocyte |
mouse myoblastoma line |
| HeLa |
human cervical adenocarcinoma cell line |
| HepG2 |
human hepatocarcinoma cell line |
| HT-29 |
human colorectal adenocarcinoma cell line |
| HUVEC |
human umbilical vein epithelial primary cell |
| MCF7 |
human breast adenocarcinoma cell line |
| MDA-MB-231 |
human breast adenocarcinoma cell line |
| NHEK-AD |
human adult keratinacyte primary cell |
| Raw264.7 |
mouse macrophage cell line |
| SW620 |
human colorectal adenocarcinoma cell line |
| U-87 MG |
human brain glioblastoma cell line |
Reagents included in each kit:
| Product # |
Product Name |
| N0538 |
N-TER Peptide, 120 µL |
| N0413 |
N-TER Buffer, 4 mL |
The amounts provided are sufficient for up to 200 reactions, assuming a final siRNA concentration of 30 nM in a final assay volume of 160 µL.
Storage/Stability
If stored properly, shelf life is 6 months from the date of shipment. The N-TER Peptide should be stored at –20 °C, and the N-TER Buffer may be stored at room temperature.
References
- Simeoni, F., et al., Insight into the mechanism of the peptide-based gene delivery system MPG: implications for delivery of siRNA into mammalian cells. Nucleic Acids Res., 31, 2717-2724 (2003).
- Morris, M.C., et al., A novel potent strategy for gene delivery using a single peptide vector as a carrier. Nucleic Acids Res., 27, 3510-3517 (1999).
- Friend, D.S., et al., Endocytosis and intracellular processing accompanying transfection mediated by cationic liposomes. Biochim. Biophys. Acta., 1278, 41-50 (1996).
- Xu, Y. and Szoka, F.C., Mechanism of DNA release from cationic liposome/DNA nanoparticles used in cell transfection. Biochemistry, 35, 5616-5623 (1996).
- Morris, M.C., et al., A new peptide vector for efficient delivery of oligonucleotides into mammalian cells. Nucleic Acids Res., 25, 2730-2736 (1997).
The N-TER Nanoparticle siRNA Transfection System is manufactured and distributed by Sigma-Aldrich under license from CNRS (France). N-TER is a trademark of Sigma-Aldrich Biotechnology
* This product is available in combination with siRNA orders.
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