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| Some of these issues of The Reporter are only available
as Adobe Acrobat files. To download or print the file, click on the icon
beside the title. To download Acrobat reader, click on the link at the bottom
of the page. |
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| Reduce Your Risk: Prevent GC Inlet Problems BEFORE
They Cost You Time and Money! |
| This Reporter discusses how gas chromatography
users will reduce the risk of chromatographic problems and instrument
downtime through regular, proper maintenance of the GC injection port.
Our technical service chemists help GC users on a daily basis to solve
problems that are the direct result of dirty liners, over used septa,
or improperly installed ferrules. This
article discusses how some simple, routine preventative maintenance
of your injection port's liner, septum, and column ferrule will prevent
big problems down the line. Be proactive with your maintenance today
or be sorry with your results tomorrow. In this issue, a case
study describes how Supelco's Technical Service helped solve a
customer's chromatography problem through GC injection port maintenance.
The Reporter also discusses
Supelco's new bulk quantity stainless steel inlet seals for Agilent
Technologies GCs and our new thermal desorption tube accessories for
PerkinElmer equipment. We also feature Supelco's full line of capillary
injector products for Agilent Technologies GCs. Our performance
tip discusses the important role of routinely inspecting and changing
your GC inlet liner to prevent reduced analyte response, poor reproducibility,
and peak tailing. Other sections
describe the new SPME troubleshooting and quantitation guides, and
our popular capillary GC troubleshooting guide. |
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| LIQUID CHROMATOGRAPHY |
19.8 |
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| New
HPLC Phases Introduced by Supelco! |
| Chemists who analyze and purify peptides
and proteins now have a choice of three new chemistries on wide pore
silica. The most novel of these phases is the C5 phase and our lead
article discusses this new phase and some applications for protein
and peptide analyses. The case
study describes the methodology for scaling up a separation from
analytical to prep using the Discovery BIO Wide Pore C18 chemistry
as an example. The new applications
section highlights the Discovery HS C18 column as the column of choice
for LC/MS. The new
products page describes all of our New Discovery HPLC phases.
In order to better service the diverse needs of today’s researcher,
each phase chemistry is available in three different particle sizes.
We are now offering Discovery BIO for biomolecule analysis and purification.
These wide pore (300Å) columns are available in three different
phase chemistries: C18, C8, and C5. In addition to BIO, the HS (120Å)
line of columns has grown to include two new unique phases in addition
to time tested C18. The entire HS line is now available in the following
bonded phase chemistries: C18, PEG, and F5. |
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| Control Your "T"s for Better
Quantitation with Solid Phase Microextraction* (SPME). |
| This Reporter discusses how to improve
reproducibility and quantitation using Solid Phase Microextraction
(SPME). By controlling and monitoring the sampling parameters discussed,
you will achieve better quantitative SPME results. In this issue,
a case study describes
how a new SPME user dramatically improved their quantitation with
the technique. The Reporter also discusses our Supelco's new
SPME StableFlex™ Fiber Assortment Kits and our new
thermal desorption tube accessories for PerkinElmer equipment.
Featured products include
recommended SPME accessories to optimize your performance and quantitation
and books on SPME and
SPE. Our performance tip
discusses the important role of flow rate in improving Solid Phase
Extraction (SPE) recoveries and reproducibility. Other
sections describe the new SPME troubleshooting and quantitation
guides, and the popular SPME CD containing the SPME Application Guide,
Third Edition, along with other SPME application notes and bulletins. |
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| LIQUID CHROMATOGRAPHY |
19.5 |
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 No
Bleed in a 3µm Liquid Chromatography/Mass Spectrometry (LC/MS)
C18 Phase! |
| Chemists who perform LC/MS analyses
benefit most from using a bleed-free column. Bleed peeks from LC/MS
columns can decrease sensitivity and may make it harder to interpret
the spectra. We introduce a new, bleed-free 3µm LC/MS column
in the lead article.
Our case study looks
at the resolution equation and how a chromatographer must consider
all three terms of the resolution equation when choosing a column.
The new applications section highlights the 3um Discovery HS (High Surface) C18 column
as the column of first choice for mass spec detection. Featured
products include both high and low pressure micro-splitters for
LC/MS. Our performance tip
includes a procedure for cleaning and regenerating a fouled column.
Finally, be sure to obtain the recently revised Pharmaceutical
Applications Guide featuring the new Discovery HS C18 column and
also request the Discovery
HS C18 Product Information Sheet. |
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| Increase Productivity by Minimizing Processing
Time Between Solid Phase Extraction (SPE) and High Performance Liquid
Chromatography (HPLC) Analysis |
| This Reporter discusses how the
use of 96-well SPE plates allows bio-analytical chemists to process
up to 96 samples at once in a single extraction. Implementing the
strategies presented will result in faster sample processing times.
In this issue, a case study relates a scenario where a customer using unconditioned SPME vial
septa created an analysis problem. The Reporter also discusses new
and innovative HPLC products, such as our new 3mm Discovery®
HS C18 columns and Discovery® SPE-96 Well Plates. Other sections
describe new literature on Solid Phase Microextraction*(SPME), the 2001 SPME Application Guide,
Third Edition, new seminars given by Supelco at PittCon 2001, and an SPME
performance tip. |
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| Selecting and Installing Injection Port Liners |
| How the Proper Installation and Selection
of Inlet Liners Leads to Better Performance of your Gas Chromatograph.
This Reporter discusses
the three areas you need to consider with injection ports liners before
the installation of a new capillary column. These are the correct
selection of liners, the cleaning of the injection port, and the proper
choice of inlet liner seals. If any of these areas are not properly
considered problems can develop; for example, broad peaks, no peaks,
too wide of a solvent peak, noisy baseline, or components being absorbed
in the inlet. If you are not careful with the installation of the
liner you will waste your time, obtain poor results, and eventually
have to repeat the installation to obtain proper operation of the
GC. In this issue, a case
study relates a scenario where a customer using the wrong design
of injection liner creates a GC problem. The Reporter also discusses
new and innovative GC products,
such as our new Thermal Desorption tubes for GERSTEL™ equipment, and
Supleco manufactured ferrules for Agilent Technologies. equipment.
Our application section
describes new literature on Solid Phase Microextraction of Odors in
Drinking Water, for Analysis by GC/MS. Also available are new
seminars given by Supelco. |
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| LIQUID CHROMATOGRAPHY |
19.2 |
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| Buffers in Reversed-Phase Liquid Chromatography |
| The selection and preparation of the
aqueous mobile phase portion in reversed-phase liquid chromatography
is of prime importance in affecting selectivity and resolution of
ionic analytes. This topic is covered in detail in the lead
article, particularly with regard to pH-buffers. Our case
study takes a look at the impact of pH on retention of some neutral,
acidic, and basic compounds, and illustrates its fundamental effect
through control of the ionization state of the analytes. The new
applications section highlights the utility of exploiting differing
column chemistry to affect selectivity. Featured
products include hardware integral to proper mobile phase preparation.
Our performance tip continues
from a previous Reporter issue, with a discussion on optimizing sample preparation for
subsequent HPLC analysis. |
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| SPME - A Fast and Inexpensive Approach to Trace Organic
Analysis |
| This issue of the Reporter addresses
two sample preparation techniques that are fast and inexpensive: SPME
for Trace Odor Analysis and SPE-96 well plates for combinatorial chemistry
applications. The main article looks at the SPME extraction of odor compounds from drinking water
at trace part per trillion concentrations. SPME provides results comparable
to the accepted closed loop stripping technique for the extraction
and detection of the compounds responsible for musty odors in drinking
water at ppt levels. The difference is that SPME takes far less time.
The Sample Preparation Performance
Tip explains how extractions with 96-well or SPE cartridges can
be improved with adjustment of sample pH. To improve retention of
basic compounds, adjust the pH of the sample matrix to at least 2
pH units above the pKa of the analyte. Reducing the pH will aid in
elution of the basic compounds. A case
study on the use of SPE-96 well plates with stryene-divinylbenzene
copolymer ion exchange sorbents was investigated for a combinatorial
chemistry application. Increased capacity of 2.5meg/g over the entire
pH range was achieved with a 75mg/well 96-well extraction platform.
The literature section
describes the new ASTM/AWWA SPME methods and Discovery SPE-96 well
plate application notes. |
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| *Solid Phase Microextraction (SPME) Technology licensed exclusively to Supelco. US patent #5,691,206; European patent #523092. |
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