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VersaFlash™ High Throughput Flash Purification |
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Sample Preparation |
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NEW! VersaFlash Videos |
Check out our line of
VersaPure™ Prepacked,
Disposable Büchner Funnels
for large-scale sample
preparation and purification. |
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Ordering Information
Additional Resources |
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| The unique, patent pending VersaFlash station and
VersaPak™ cartridge designs combine to make a truly novel flash
purification system that saves time and money while allowing you to
do things that other systems don’t. VersaFlash saves time and
money by allowing:
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Here are two great opportunities
to learn more about our
VersaFlash High Throughput Flash Purification System.
We will gladly send to you a VersaFlash Brochure or a VersaFlash
CD (which includes videos demonstrating the features and use
of the VersaFlash system). We can also schedule a demonstration
of the VersaFlash system.
Click on the image to request a brochure, CD, or demo.
(more resources below) |
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| Easy scale-up and full cartridge range
compatibility |
| In less than one minute you can scale-up from the
smallest cartridge to the largest cartridge giving you the flexibility
you need to handle a wide range of sample sizes. Many flash chromatography
systems limit users to a narrow sample range requiring different hardware
purchase and laborious change-out in order to work with larger sample
sizes. The robust VersaPak cartridge design eliminates the need for
a compression barrel making cartridge change-over and scale-up fast
and easy. The VersaFlash system gives you the versatility to work
with discovery scale cartridges (40 x 75mm) and requiring only a simple
adjustment of the upper platen to work with development scale cartridges
(80 x300mm). |
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| Figure 1. |
VersaFlash is Scalable Across the Entire
Range of Cartridge Sizes Using the Exact Same Hardware |
| 1a. |
To switch to a larger cartridge, simply turn the
handle counter-clockwise to the idle position and pull it out
lowering the bottom sealing platen. |
| 1b. |
Once the cartridge is removed, push the handle
in, loosen the securing knob on the upper platen and raise it
to the proper height to insert the larger cartridge. |
| 1c. |
With the upper platen resting on the cartridge,
tighten the securing knob. |
| 1d. |
Turn the handle clockwise toward the operate position
sealing the cartridge in the VersaFlash station. |
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| Fast and easy cartridge change-over |
| The unique VersaPak pre-compression
design eliminates the need for compression barrels and nitrogen pressurization
making cartridge change-over fast and easy. Installing, removing and
changing over VersaPak cartridges in the VersaFlash station is fast
and easy. |
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| Figure 2. |
Fast and easy cartridge change-over |
| 2a. |
You can see the previously used cartridge sealed
into the VersaFlash station with the handle in the operate position. |
| 2b. |
Turn the handle counter-clockwise to the idle
position and pull it out to remove the used cartridge. |
| 2c. |
Insert a new cartridge, push the handle in and
turn it clockwise toward the operate position to seal the new
cartridge. |
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| Compatiblity with most flash solvent
delivery systems |
| The VersaFlash can be purchased as
a complete system with the Supelco pump or you can connect it to most
existing solvent delivery systems to begin using the many capabilities
of the VersaFlash station. |
| Cartridge stacking for bi-modal separations |
| An additional benefit of the unique
design of the VersaPak cartridges and the VersaFlash station is that
it allows you to stack multiple cartridges together. Two cartridges
with similar media can be stacked to increase the loading capacity
or two cartridges with different media (silica over C18) can be stacked
for complex bi-modal separations. |
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| Figures 3a-c. |
Efficient Separation of a Multi-Dye Mixture
Using BiModal Stacked Cartridges (Silica and C18) |
| 3a. |
The dye mixture (Red 40, Blue 1 and Yellow 5) is loaded
on the bottom of the cartridge and begins to separate on the cartridges. |
| 3b. |
The yellow 5 dye elutes first with water. |
| 3c. |
The red 40 dye then elutes with water. |
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| Rev-Elution for
faster component elution |
| When desired compounds are fully retained
on the cartridge, they must be eluted using a stronger mobile phase.
Normally this spreads the band as it passes through the full length
of the media bed. In addition if there are traces of other components
remaining on the cartridge, contamination of the retained component
is more likely to occur. Finally excess solvent is required to move
the band entirely through the cartridge. These problems are solved
by Rev-elution, or the technique of reversing the cartridge after
partial separation has occurred. Consequently the retained substance
can be quickly and cleanly eluted using a slightly stronger mobile
phase without the need to travel through the entire cartridge bed.
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| Figures 3d-e. |
Efficient Separation of a Multi-Dye Mixture
Using Rev-Elution |
| 3d. |
After removing the (upper) silica cartridge, the C18
cartridge is reversed putting the blue dye at the cartridge outlet
(top). |
| 3e. |
The blue 1 dye then elutes more quickly using 100% methanol
without traveling the entire length of the cartridge bed. |
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| Flexible sample loading options |
The VersaPak cartridge allows you a
wide variety of ways to load samples on the cartridge. There are at
least five ways to load a sample onto a VersaPak cartridge including:
- Direct sample injection onto the cartridge using a syringe (off-line)
- Vacuum aspiration onto the cartridge using the VersaVac sample
loading station (off-line)
- Using a solid sample cartridge prior to the primary cartridge
(on-line)
- Using a valve and loop injector for repetitive volume sample
loading (on-line)
- Through the pump loading for dilute samples (on-line)
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| Figure 4. |
Direct Sample Loading onto Cartridge |
| Figure 5. |
Loading Multiple VersaPak Cartridges |
| Figure 6. |
Partial Separation |
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| Figure 7. |
Separation of a Chlorophyll/Carotenoid Mixture Using
One-Step
Pre-Purification |
| 7a. |
Load sample on C18 cartridge using silica SPE tube
for pre-purification. |
| 7b. |
Carotenoids were eluted into the VersaPak with methanol/dichloromethane,
chlorophyll sticks on the silica SPE cartridge (green band). |
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VersaFlash station
The VersaFlash station secures the VersaPak cartridges for processing. It
is available separately or in a complete system.
| Specifications |
| Height: |
70cm (27.5 in.) |
| Length: |
28cm (11 in.) |
| Width: |
21cm (8 in.) |
| Weight: |
12kg (27 lbs.) |
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| Description |
Cat.
No. |
VersaFlash station assembly  |
97732-U |
VersaFlash System 1 (220 Volt)
(includes VersaFlash station, pump, VersaVac sample loading station
and Starter Kit) |
97720-U |
VersaFlash System 1 (110 Volt)
(includes VersaFlash station, pump, VersaVac sample loading station
and Starter Kit) |
97730-U |
VersaPak cartridges
A key feature of the VersaPak cartridges is the patent-pending end-fitting
design. The symmetric end-fitting design allows for fast and easy cartridge
change-out, cartridge stacking, rev-elution and flexible sample loading
methods. There is no need for compression barrels or external compressed
gas supplies since the cartridges are pre-compressed and sealed by the end-fittings.
VersaPak cartridges are tightly held in place during pressurization by the
leak-resistant VersaFlash station.
Pump
Most flash chromatography systems utilize a pressurized stainless steel
reservoir to contain the solvent and force it through the flash cartridges.
The VersaFlash system uses a pump eliminating the need for a stainless steel
reservoir, thereby eliminating the following limitations:
- The level of solvent cannot be seen often times causing incomplete
separations and unknown solvent flush volumes
- The reservoir is difficult to empty, clean and refill any time solvent
change is required.
- Solvent volume is limited by the tank capacity.
- An external gas supply is required to pressurize the tank.
| Description |
Cat.
No. |
| Assembled Pump, 120V |
97734-U |
| Assembled Pump, 220V |
97737-U |
Kits and Accessories
VersaVac Sample Loading Station
The VersaVac sample loading station is a vacuum manifold designed to aid
in the loading of samples onto the VersaPak cartridges. From one to six
(40mm) cartridges can be loaded on the VersaVac at one time.
| Description |
Cat.
No. |
| VersaVac Sample Loading Station |
97750-U |
Solid sample cartridges
Solid sample cartridges allow you to adsorb samples onto silica or other
packing materials and pack the dried sample into the solid sample cartridge
and link it to the separation cartridge, allowing for direct desorption
by the mobile phase. Samples that have low solubility in the mobile phase
can be transferred to the head of the cartridge with no tubing or fittings
to restrict or clog due to particulate.
| Description |
Quantity |
Cat.
No. |
| Solid Sample Cartridge
Kit |
6 small and
6 large Solid sample cartridges |
97734-U |
| Small (40 X 37.5
mm) solid sample cartridges |
12/pk |
97746-U |
| Large (40 x 75 mm)
solid sample cartridges |
12/pk |
97747-U |
Starter Kit
The VersaFlash starter kit is designed to provide you with everything that
you will need to get started using the capabilities of the VersaFlash system.
From flushing solvents out of the system to stacking multiple cartridges.
The kit includes a 40 x 40 mm cartridge stacking assembly, a solvent
flush connector, 1/8” tubing (10 ft.), and solid sample cartridges
(3 small and 3 large).
| Description |
Cat.
No. |
| Starter Kit |
97739-U |
Cartridge stacking assemblies
VersaFlash cartridge stacking assemblies allow you to stack multiple cartridges
together to increase the sorbent capacity and/or to combine different phase
chemistries to conduct bi-modal separations. Cartridge stacking assemblies
are available in three sizes: 40 x 40 mm, 40 x 80 mm, and 80 x 80 mm to
stack different diameter cartridges.
| Description |
Cat.
No. |
| Cartridge Stacking Assembly (40 x 40
mm) |
97740-U |
| Cartridge Stacking Assembly (40 x 80
mm) |
97741-U |
| Cartridge Stacking Assembly (80 x 80
mm) |
97742-U |
Solvent Flush Connector
The solvent flush connector I used to flush solvents out of the VersaFlash
station or when changing from one solvent to another without a VersaPak
cartridge in the VersaFlash station.
| Description |
Cat.
No. |
| Solvent Flush Connector |
97743-U |
| Title |
Publication
ID. |
| VersaFlash Key Features Handout |
T403130 |
| VersaFlash Operating Manual |
T703012 |
| VersaFlash Cartridges (data sheet) |
T703008 |
| VersaFlash Solid Sample Cartridges
(data sheet) |
T703009 |
| VersaFlash Starter Kit (data sheet) |
T703014 |
| VersaFlash PEEK Seal Assembly (data
sheet) |
T703021 |
| Instruction Sheet for Priming the Pump
in the VersaFlash System |
T704004 |
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