Probe based Quantitative PCR

Probe based QPCR relies on the sequence–specific detection of a desired PCR product. Unlike SYBR based QPCR methods that detect all double–stranded DNA, probe based QPCR utilizes a fluorescent–labeled target-specific probe resulting in increased specificity and sensitivity. Additionally, a variety of fluorescent dyes are available so that multiple primers can be used to simultaneously amplify many sequences.

• Ideal for high throughput. ReadyMixes contain all necessary components for QPCR you simply add the fluorescent detection chemistry, primers, and template.
• Specific – JumpStart Taq antibody prevents non–specific product formation through it hot–start mechanism.
• Maximum flexibility in detection method, since no detection method has been incorporated into formulation.
• Optimized formulations with the addition of dUTP to facilitate carry–over prevention from previous PCR.

Product Name	Technical Bulletin	Product #
JumpStart Taq ReadyMix for for High Throughput QPCR		D6442
JumpStart Taq ReadyMix for QPCR		D7440
JumpStart Taq ReadyMix with dUTP		D9191

Mechanism of probe based protocol

Figures 1 & 2. Superior Amplification Efficiency with Lower CT.

Probe–based real–time PCR, dual labeled probes and Molecular Beacons are available from Sigma Genosys.

Sigma-Aldrich Teams with Epoch Biosciences
Sigma-Aldrich has teamed with Epoch Biosciences to create an optimized PCR reagent for the MGB Eclipse™ Probe Systems for both Gene Expression Profiling and SNP Analysis. The PCR reagents are pre-formulated with necessary buffers and deoxynucleotides and when combined with JumpStart™ Taq DNA Polymerase creates a ready-to-use 2X PCR master mix.

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