|
Move Beyond Traditional PCR Limitation...Unsurpassed Yield,
Unlimited Potential
The GenomePlex® Whole Genome Amplification (WGA) family of products provides a robust and accurate method of amplifying nanogram quantities of starting material into microgram yields with minimal allele drop out.
The GenomePlex WGA product line utilizes a proprietary amplification technology based upon random fragmentation of genomic DNA and conversion of the resulting small fragments to PCR-amplifiable OmniPlex® Library molecules flanked by universal priming sites. The OmniPlex library is then PCR amplified using universal oligonucleotide primers and a limited number of cycles.
WGA has been used in a variety of applications,1, 2, 3 and is suitable for use with purified genomic DNA from a variety of sources including blood cards, whole blood, buccal swabs, soil, plant, and formalin-fixed paraffin-embedded (FFPE) tissues. GenomePlex WGA uses nanogram quantities of starting genomic DNA, which after PCR yields 5 to 10 µg of WGA product. After purification, the WGA product can be analyzed in a manner similar to any genomic or chromosomal DNA sample. A number of downstream applications may be performed including TaqMan® assays, CGH analysis, SNP analysis, sequencing, etc.
- Choose from a variety of DNA sources: whole blood, blood card, plasma, serum, buccal swab, plant, soil, formalin-fixed paraffin-embedded (FFPE) tissue and single cells
- See a complete representation of the entire genome with no detectable allele bias.
- Increase amplification accuracy with WGA DNA Polymerase
- Preserve precious source material by amplifying nanogram amounts of starting genomic DNA into microgram yields in a few hours
- Enjoy the compatibility GenomePlex provides with many downstream applications: TaqMan assays, SNP analysis, microarray analysis, sequencing, or store at -20 °C for future use
|
References
- Little, S.E., et al., Array CGH using whole genome amplification of fresh-frozen and formalin-fixed, paraffin-embedded tumor DNA. Genomics. Oct 31, 2005.
- Barker, D. L., et al., Two methods of whole-genome amplification enable accurate genotyping across a 2320-SNP linkage panel. Genome Res., 14, 901-907 (2004).
- Gribble, S., et al., Chromosome paints from single copies of chromosomes. Chromosome Res., 12, 143-151 (2004).
- Thorstenson, Y. R., et al., An Automated Hydrodynamic Process for Controlled, Unbiased DNA Shearing. Genome Res., 8, 848-855 (1998).
- Park, J.W., et al., Comparing Whole-Genome Amplification Methods and Sources of Biological Samples for Single-Nucleotide Polymorphism Genotyping. Clinical Chemistry., 51(8), 1520-1523 (2004).
back to the Whole Genome Amplification home page
|
