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Spent Medium Analysis |
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Cell Culture |
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SAFC delivers
analytical expertise critical to your medium development process. Using spent
medium analysis, we'll help you determine formula changes that
lead to productivity improvements. The result? Increased medium performance
and ultimately, a higher return on investment for you and your project
For most analyses, i.e., amino acids, vitamins, trace elements, etc., a 5
mL sample size for each assay is sufficient.
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Cell culture spent medium samples
must be prepared prior to testing by separating cells and cellular debris
from the medium: |
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Harvest cells and media aseptically into 15 mL conical tubes. |
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Spin down media at 2600g for 5 minutes. This step
insures that no cellular debris interferes with the analysis. |
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Transfer spent medium supernatant
into labeled containers (identified with your company/institution, date,
experiment #, and days of incubation) that can be frozen at –20 °C
for storage and shipment. |
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Spent medium samples must be shipped
on dry ice. |
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Please
use this form when sending samples. Please
provide sufficient notice before sending samples by contacting Dr. Ben
Cutak, Senior R&D Scientist, at 314 289-8496, extension
1961,
e-mail at: bcutak@sial.com. |
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Analysis
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Methods
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Pricing
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Expected Turn–Around Time
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Amino Acids
Current Assays: ASP, GLU, HYP, SER, ASN, GLY, GLN, HIS, TAU, THR, ARG,
ALA, PRO, TYR, CYS2, VAL, MET, ILE, LEU, LYS, PHE, TRP |
HPLC with Fluorescence Detection
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$120/sample
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2 weeks from receipt of samples
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IgG Analysis
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Affinity Chromatography
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$450 for 1 to 40 samples
Additional charges will be incurred for greater than 40 samples
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2 weeks from receipt of samples
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Glucose/Lactate and Glutamine/Glutamate Analysis
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WSI Biochem Analyzers
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$25/sample
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1 week from receipt of samples
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Water–Soluble Vitamins
Currrent Assays: Pantothenic Acid, Pyridoxine, Thiamine, Niacinamide,
Folic Acid, Vitamin B12, Riboflavin |
HPLC with UV and MS Detection
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$335/sample
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2 weeks from receipt of samples
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Elemental Analysis
Note: Elements at concentrations <10 µg/ml require different
instrumentation and additional set–up time |
ICP
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$450/sample for analysis of elements at concentrations >10 µg/ml
$750/sample for analysis of elements at concentrations <10 µg/ml
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2 weeks from receipt of samples
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