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New Proligo® Reagents

D8001

New Products:
	2’Fluoro Phosphoramidites


	5’-NPPOC Phosphoramidites

2’Fluoro Phosphoramidites

2’Fluoro Phosphoramidites are used to synthesize oligonucleotides that are more thermally stable and provide increased nuclease resistance.

Features of 2’Fluoro Phosphoramidites:

	Can be employed together with DNA or RNA phosphoramidites
	Recommended deprotection conditions are 8 hours at 55°C using concentrated ammonia solution, or with AMA for 10 minutes at 65°C
	Synthesis of 2’Fluoro oligonucleotides is similar to standard DNA synthesis, but requires an elongated coupling time (recommended is 3 minutes compared to 90 seconds for DNA monomers)
	Lot-to-lot consistent high purity and performance
	Manufactured under a certified ISO 9001 quality system

Test	2’Fluoro-dC(ac)	2’Fluoro-dU
HPLC Purity	≤98.0%	≤98.0%
³¹P-NMR	≤99%	≤99%

Phosphoramidites
Product Information
Product No.	Description	Unit
C21328-HH	DMT-2’Fluoro-dC(ac) Amidite - PerkinElmer 8900 & Polygen	1 x 0.25g
C21323-HH	DMT-2’Fluoro-dC(ac) Amidite, ABI	1 x 0.25g
C21323-HH	DMT-2’Fluoro-dC(ac) Amidite, ABI	1 x 1g
U21128-HH	DMT-2’Fluoro-dU Amidite - PerkinElmer 8900 & Polygen	1 x 0.25g
U21123-HH	DMT-2’Fluoro-dU Amidite, ABI	1 x 0.25g
U21123-HH	DMT-2’Fluoro-dU Amidite, ABI	1 x 1g

Enquire about delivery dates of these specialty amidites.

5’-NPPOC Phosphoramidites

Phosphoramidites with a NPPOC 5’-protection group can be used for the production of DNA microarrays using photolithographic in situ synthesis of oligonucleotides directly on the chip. This method offers the possibility to produce DNA chips of extremely high density and provides a flexible system.

Features of NPPOC Chemistry:

	Efficient photolytic deprotection (> 99%)
	Less side products during photolysis
	Products available
	Lot-to-lot consistent high purity and performance
	Manufactured under a certified ISO 9001 quality system

Analytical Specifications Test	A	C	G	T
HPLC Purity	≤ 96.0 %	≤96.0 %	≤96.0 %	≤96.0 %
³¹P-NMR	≤96.0 %	≤96.0 %	≤96.0 %	≤96.0 %

Phosphoramidites
Product Information
Product No.	Description	Unit
A112N01-01	NPPOC-dA(tac) Amidite	1 x 1g
C114N8-HH	NPPOC-dC(ib) Amidite	1 x 1g
G114N3-HH	NPPOC-dG(ipac) Amidite	1 x 1g
T111N8-HH	NPPOC-dT Amidite	1 x 1g

Enquire about delivery dates of these specialty amidites.

User Instructions

1. Liquid Reagents

Deblock - The NPPOC-protective group is removed with light while the support-anchored oligonucleotide is immersed in a liquid reagent; various formulations are applicable, our recommended formulations are 1% NMI in DMSO (v/v) or 1% NMI in ACN (v/v) Activator - Any commercial activator that works for DNA-amidites is applicable; 0.25M DCI in ACN is perfect.

Oxidizer - Although any commercial oxidizer is applicable some customers use flow cells that are incompatible with THF and therefore prefer to use an oxidizer wherein the solvent THF is replaced by ACN.

Cap A - Fast Cap A is recommended (TAC-anhydride solution); some customers use flow cells that are incompatible with THF and therefore prefer to use a Cap A reagent wherein the solvent THF is replaced by ACN; some customers do not employ capping steps with NPPOC-chemistry.

Cap B - Cap B solutions that are compatible with Fast Cap A; some customers use flow cells that are incompatible with THF and therefore prefer to use a Cap B reagent wherein the solvent THF is replaced by ACN.

2. Coupling

NPPOC-amidites are dissolved in ACN at the concentration recommended for standard DNA amidites by the manufacturer of the DNA/RNA synthesizer. The coupling times of NPPOC-Amidites match the coupling times of standard DNA amidites (30 to 60 sec., longer coupling times are applicable, but not necessary).

3. Deprotection of NPPOC-groups

Light with a wavelength between 350 and 390 nm is applicable. The deprotection time depends on the light intensity. 90 sec. illumination time is sufficient at 100mW/cm2 on the surface of the synthesis support (365 nm Hg/Xe high pressure lamp).

4. Deprotection of base protective groups

Any fast deprotection protocol (e.g. conc. ammonia/room temp./2 hours or AMA/room temp./30 min.) is applicable. However, such treatment will partly remove the oligonucleotides from silica surfaces and therefore a milder treatment with a mixture of ethylendiamine and ethanol 50/50, v/v, for 2 hours at room temperature is used as an industry standard.