| Biotin-labeled oligonucleotides have become extremely popular in DNA capture and detection applications, ude to their very high sensitivity and strong, specific binding affinity with avidin and streptavidin proteins. Bioten-based detection methods are widely used by reserchers. Biotin labeling is compatible with PCR and most hybridization techniques. |
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| Key Features of the Biotin Phosphoramidite |
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Behaves like any standard amidite on the DNA synthesizer |
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Readily soluble in acetonitrile |
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Biotin compound has a dimethozytrityl (DMT) - group on the ring structure, which allows coupling-yeild determination and trityl-selective purification |
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A long, water-compatible linker arm ensures maximum sensitivity |
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The labeled oligonucleotide is ready for use in most applications after the evaporation of ammonia. Standard procedures can be used if additional purification is required |
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Provides a couplin efficiency of 95% |
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