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Liquid Reagents |
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Proligo® Reagents |
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| Product Breadth of Proligo Reagents |
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| Product Breadth of Proligo Reagents |
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| In solid-phase synthesis of oligonucleotides, liquid reagents are used in each step of the synthesis cycle. Overall synthesis performance, and therefore total product yield and purity of the crude oligonucleotide, is highly dependent on the chemical purity of the monomers and the supporting liquid reagents. |
| SAFC Proligo Reagents has more than 20 years of manufacturing experience in DNA and RNA synthesis reagents. Our liquid reagents meet the most stringent quality specifications, making them ideal for use in automated nucleic acid synthesis. Lot-to-lot consistency is guaranteed for every product manufactured in our ISO 9001 certified site. Each lot is individually quality-controlled to ensure optimum synthesis performance. |
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The Oligonucleotide Synthesis Cycle
(Click on image for larger view)
For TAC-protected G-amidites (dG(tac) phoshoramidite, rG(tac) phosphoramidite) Fast Deprotection Cap A must be employed to prevent transacylation side reactions on the guanine base.
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Chemical Composition and Purpose of Proligo Reagents’ Liquid Reagents
During the Synthesis Cycle |
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Deblock solution, containing trichloracetic acid (TCA) or dichloroacetic acid (DCA) in dichloromethane, removes the dimethoxytrityl (DMT) protecting group from the 5’ hydroxyl moiety of nucleotides already incorporated into the growing nucleic acid, prior to the addition of the next phosphoramidite. Removal of the DMT allows the unprotected 5’ hydroxyl moiety to react with a new phosphoramidite in a subsequent extension reaction. |
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Wash solution, containing anhydrous acetonitrile (water content ≤ 30ppm), is used as the overall wash solvent following each step in the synthesis cycle. |
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Activator solution, containing powerful activators like 4,5-dicyanoimidazole (DCI) or 5-(3,5-bis(trifluoromethyl)phenyl)-1H-tetrazole (Activator 42™) in acetonitrile, are mixed with solutions of phosphoramidites during the extension step. The activator reacts with the amidite group to form a highly reactive intermediate. The intermediate then forms an internucleotide bond with the detritylated 5’-hydroxyl group of the growing oligonucleotide chain. |
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Cap A solution, containing acetic anhydride and tetrahydrofuran (THF), is used after the phosphoramidite reaction/coupling step. This solution aborts chains bearing unreacted 5’hydroxyl groups due to failure of reaction with activated phosphoramidite (typically, 1 to 2% of growing oligonucleotide chains in each synthesis cycle). These unreacted 5’hydroxyl groups are capped with an acetyl group and thus rendered unreactive for subsequent synthesis steps. For synthesis with Proligo Reagents’® TAC-protected phosphoramidites, Fast Deprotection CAP A solution must be employed instead of Cap A solution. |
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Fast Deprotection Cap A solution, must be used for synthesis with Proligo Reagents’® TAC-protected phosphoramidites. Fast Deprotection Cap A solution, containing tertbutylphenoxyacetyl acetic anhydride (tac2O) in tetrahydrofuran, is used in place of Cap A solution to ensure that the displacement of tert-butylphenoxyacetyl (TAC) on guanine bases of the TAC-protected RNA phosphoramidites does not occur. |
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Cap B solution, containing tetrahydrofuran (THF), pyridine and N-methylimidazole (NMI), is mixed in-situ with Cap A solution during the capping (acetylation) reaction. Pyridine is applied as a mild base while NMI provides a powerful acylation catalyst. |
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Oxidizer solution, containing tetrahydrofuran (THF), pyridine, iodine and water, is applied to oxidise the chemically unstable trivalent phosphorous triester linkage formed in the coupling reaction to a stable pentavalent phosphate triester. Iodine functions as a mild oxidant and water functions as an oxygen donor. |
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| Liquid Reagents |
| Product No. |
Description |
Unit |
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ACN; Methyl cyanide
CH3CN FW 41.05 [75-05-8] |
| L010010-06 |
Amidite Diluent (Acetonitrile) |
6 x 100ml |
| L010250-04 |
Acetonitrile |
4 × 2.5 L |
| L010400-04 |
Acetonitrile |
4 × 4 L |
| L015000-01 |
Acetonitrile |
50 L |
| L015000-01 |
Acetonitrile |
50 L |
| L010010-06 |
amidite diluent |
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| L020250-04 |
TCA deblock |
4 × 2.5 L |
| L020400-04 |
TCA deblock |
4 × 4 L |
| L023000-01 |
TCA deblock |
30 L |
| L020251-04 |
DCA deblock |
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| L080250-04 |
DCI activator -- 0.25 M |
4 × 2.5 L |
| L080400-04 |
DCI activator -- 0.25 M |
4 × 4 L |
| L083000-01 |
DCI activator -- 0.25 M |
30 L |
| L340018-06 |
Cap A |
6 × 180 mL |
| L340045-06 |
Cap A |
6 × 450 mL |
| L040251-01 |
Cap A |
2.5 L |
| L040250-01 |
Cap A |
2.5 L |
| L040400-01 |
Cap A |
1 × 4 L |
| L350018-06 |
Cap B |
6 × 180 mL |
| L350045-06 |
Cap B |
6 × 450 mL |
| L050251-01 |
Cap B |
2.5 L |
| L050250-01 |
Cap B |
2.5 L |
| L050400-01 |
Cap B |
1 × 4 L |
| L060250-04 |
Oxidizer -- 0.02 M |
4 × 2.5 L |
| L060252-04 |
Oxidizer -- 0.02 M |
4 × 2.5 L |
| L060400-04 |
Oxidizer -- 0.02 M |
4 × 4 L |
| L070250-01 |
Fast deprotection Cap A |
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| L0302502-04 |
Activator 42™ 0.1M |
4 x 2.5L |
| L8300452-06 |
Activator 42™ 0.1M |
6 x 450ml |
| L8300202-06 |
Activator 42™ 0.1M |
6 x 200ml |
| L0302512-04 |
Activator 42™ 0.25M |
4 x 2.5L |
| L8300462-06 |
Activator 42™ 0.25M |
6 x 450ml |
| L8300212-06 |
Activator 42™ 0.25M |
6 x 200ml |
| Liquid Reagents |
| ACN Bases Liquid Reagents |
| Product No. |
Description |
Unit |
| LB40250-01 |
Cap A -- acetonitrile solution |
1 x 2.5L |
| LB50250-01 |
Cap B -- acetonitrile solution |
1 x 2.5L |
| LR40250-01 |
Cap 1 -- acetonitrile solution |
1 x 2.5L |
| LR50250-01 |
Fast Deprotection Cap 2 -- acetonitrile solution |
1 x 2.5L |
| LR60250-04 |
Oxidizer -- acetonitrile solution |
4 x 2.5L |
| Liquid Reagents |
| Compatible with Expedite™ and Polygen® Instruments |
| Product No. |
Description |
Unit |
| L840020-06 |
Cap A |
6 × 200 mL |
| L840045-06 |
Cap A |
6 × 450 mL |
| L850020-06 |
Cap B |
6 × 200 mL |
| L850045-06 |
Cap B |
6 × 450 mL |
| L370018-06 |
Fast deprotection Cap A |
6 × 180 mL |
| L370045-06 |
Fast deprotection Cap A |
6 × 450 mL |
| L870020-06 |
Fast deprotection Cap A |
6 × 200 mL |
| L860020-06 |
Oxidizer -- 0.02 M |
6 × 200 mL |
| L860045-06 |
Oxidizer -- 0.02 M |
6 × 450 mL |
| L820090-06 |
TCA deblock |
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| L880020-06 |
DCI Activator 0.25M |
6 × 200 ml |
| L880045-06 |
DCI Activator 0.25M |
6 × 450 ml |
| Liquid Reagents |
| Compatible with ABI™ Instruments |
| Product No. |
Description |
Unit |
| L340018-06 |
Cap A |
6 × 180 mL |
| L340045-06 |
Cap A |
6 × 450 mL |
| L040251-01 |
Cap A |
2.5 L |
| L350018-06 |
Cap B |
6 × 180 mL |
| L350045-06 |
Cap B |
6 × 450 mL |
| L050251-01 |
Cap B |
2.5 L |
| L380018-06 |
DCI activator -- 0.25 M |
6 × 180 mL |
| L380045-06 |
DCI activator -- 0.25 M |
6 × 450 mL |
| L370018-06 |
Fast deprotection Cap A |
6 × 180 mL |
| L370045-06 |
Fast deprotection Cap A |
6 × 450 mL |
| L860021-06 |
Oxidizer -- 0.02 M |
6 × 200 mL |
| L260045-06 |
Oxidizer -- 0.02 M |
6 × 450 mL |
| L360020-06 |
Oxidizer -- 0.1 M |
6 × 200 mL |
| L360045-06 |
Oxidizer -- 0.1 M |
6 × 450 mL |
| L320045-01 |
TCA deblock |
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| Compatible with Äkta™ and Oligo Pilot™ Instruments |
| Product No. |
Description |
Unit |
| L520251-04 |
DCA Deblock (Toluene) |
4 × 2.5 L |
| L540250-01 |
Cap A for Äkta™ and Oligo Pilot™ |
1 × 2.5 L |
| L550250-01 |
Cap B1 for Äkta™ and Oligo Pilot™ |
1 × 1.25 L |
| L550251-01 |
Cap B2 for Äkta™ and Oligo Pilot™ |
1 × 1.25 L |
| L560250-04 |
Oxidisier 0.05M for Äkta™ and Oligo Pilot™ |
4 × 2.5 L |
| Custom products |
Please inquire for customized liquid reagents with specialty |
| Bulk |
Packaging in drums or m3-containers is available upon request. |
SAFC and SAFC Proligo Reagents are trademarks of Sigma-Aldrich Biotechnology, LP.
©2006 Sigma-Aldrich Biotechnology LP. All rights reserved. Reproduction forbidden without permission.
Other trademarks are the property of their respective owners. |
To order or speak with a Customer Service Representative,
contact your local SAFC office. |
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