It is possible to effect an enantiomeric separation using conventional HPLC and CE stationary phases by adding a chiral selector to the mobile phase. Chiral selector additives generally interact via ion pair, ligand exchange, or inclusion interactions with enantiomer analytes, forming mobile diastereomeric complexes that are separable by conventional normal phase or reversed-phase columns. When free cyclodextrins are added to the mobile phase, inclusion complexes are formed and separations can approach those obtained on cyclodextrin-based chiral stationary phases (CSPs). Note - this approach often leads to a reversal in the elution order obtained on a cyclodextrin CSP. Sigma-Aldrich carries a number of high-purity cyclodextrin derivatives for this application.
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