TSKgel® Anion Exchange HPLC Columns

Ion exchange chromatography separates molecules based on differences in the net charge of the molecules. In anion exchange, the analyte is negatively charged (anion), while the chromatographic material is positively charged (cation). Ion exchange is commonly used for protein purification, but it may be used for purification of oligonucleotides, peptides, or other charged molecules. For interaction to occur, the protein of interest must have a charge opposite to that of the functional group of the sorbent particle. Because the interaction is ionic, binding must take place under low ionic strength conditions. Elution is achieved by increasing the ionic strength of the mobile phase to reduce ionic attractions, or by changing the pH of the mobile phase to alter the ionization state of the protein.

TSKgel columns are highly efficient for sample purification with excellent recovery. Anion- and cation-exchangers are available on porous polymer-based and silica-based particles. Proteins and nucleic acids can be analyzed faster on a TSKgel non-porous resin column, although STAT and NPR columns have lower capacity than large-pore-size-polymer particles. TSKgel BioAssist columns are constructed from PEEK (polyether ether ketone).

TSKgel® DEAE-5PW Columns (1000Å pore size)

Product #

Description

Phase

Particle Size

Length × I.D.

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807210 TSKgel® DEAE-5PW Guardgel Kit DEAE (diethylaminoethyl) phase
20 μm
2.5 cm × 6 mm
816092 TSKgel® DEAE-5PW Guardgel Kit DEAE (diethylaminoethyl) phase
20 μm
3.5 cm × 10 mm
814466 TSKgel® DEAE-5PW Guards and Guardgel Kits DEAE (diethylaminoethyl) phase
13 μm
2 cm × 20 mm
808806 TSKgel® DEAE-5PW Guards and Guardgel Kits DEAE (diethylaminoethyl) phase
20 μm
1 cm × 8 mm
814016 TSKgel® DEAE-5PW HPLC Column DEAE (diethylaminoethyl) phase
13 μm
15 cm × 20 mm
813061 TSKgel® DEAE-5PW HPLC Column DEAE (diethylaminoethyl) phase
10 μm
5 cm × 5 mm
808802 TSKgel® DEAE-5PW HPLC Column DEAE (diethylaminoethyl) phase
10 μm
7.5 cm × 8 mm
807164 TSKgel® DEAE-5PW HPLC Column DEAE (diethylaminoethyl) phase
10 μm
7.5 cm × 7.5 mm
807574 TSKgel® DEAE-5PW HPLC Column DEAE (diethylaminoethyl) phase
13 μm
15 cm × 21.5 mm
818757 TSKgel® DEAE-5PW HPLC Column DEAE (diethylaminoethyl) phase
10 μm
7.5 cm × 2 mm

TSKgel® SuperQ-5PW Columns (1000Å pore size)

Product #

Description

Phase

Particle Size

Length × I.D.

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818388 TSKgel® SuperQ-5PW Guardgel Kit ammonium ion, quaternary phase
20 μm
2.5 cm × 6 mm
818257 TSKgel® SuperQ-5PW HPLC Column ammonium ion, quaternary phase
10 μm
7.5 cm × 7.5 mm
818387 TSKgel® SuperQ-5PW HPLC Column ammonium ion, quaternary phase
13 μm
15 cm × 21.5 mm
818386 TSKgel® SuperQ-5PW HPLC Column ammonium ion, quaternary phase
10 μm
7.5 cm × 8 mm

TSKgel® BioAssist® Q Columns (4000Å pore size)

Product #

Description

Phase

Particle Size

Length × I.D.

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819685 TSKgel® BioAssist® Q HPLC Column polyamine phase
10 μm
5 cm × 4.6 mm
821410 TSKgel® BioAssist® Q HPLC Column polyamine phase
13 μm
10 cm × 10 mm

TSKgel® NPR Columns (non-porous)

Product #

Description

Phase

Particle Size

Length × I.D.

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818253 TSKgel® DEAE-NPR Guard Column DEAE (diethylaminoethyl) phase
2.5 μm
0.5 cm × 4.6 mm
813075 TSKgel® DEAE-NPR HPLC Column DEAE (diethylaminoethyl) phase
2.5 μm
3.5 cm × 4.6 mm
817088 TSKgel® DNA-NPR Guard Column DEAE (diethylaminoethyl) phase
5 μm
0.5 cm × 4.6 mm
818249 TSKgel® DNA-NPR HPLC Column DEAE (diethylaminoethyl) phase
2.5 μm
7.5 cm × 4.6 mm

TSKgel® STAT Columns (non-porous)

Product #

Description

Phase

Particle Size

Length × I.D.

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821962 TSKgel® DNA-STAT HPLC Column ammonium ion, quaternary phase
5 μm
10 cm × 4.6 mm
821960 TSKgel® Q-STAT HPLC Column ammonium ion, quaternary phase
10 μm
3.5 cm × 3 mm
821961 TSKgel® Q-STAT HPLC Column ammonium ion, quaternary phase
7 μm
10 cm × 4.6 mm

Silica-based TSKgel® Anion Exchange Columns

Product #

Description

Phase

Particle Size

Length × I.D.

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818761 TSKgel® DEAE-SW Columns DEAE (diethylaminoethyl) phase
5 μm
25 cm × 2 mm
807168 TSKgel® DEAE-SW Columns DEAE (diethylaminoethyl) phase
5 μm
25 cm × 4.6 mm
807163 TSKgel® DEAE-SW Columns DEAE (diethylaminoethyl) phase
10 μm
7.5 cm × 7.5 mm
807648 TSKgel® DEAE-SW Guardgel Kit DEAE (diethylaminoethyl) phase
20 μm
2.5 cm × 6 mm
842154 TSKgel® DEAE-SW Guardgel Kit DEAE (diethylaminoethyl) phase
5 μm
1 cm × 2 mm
807646 TSKgel® QAE-2SW Guardgel Kit ammonium ion, quaternary phase
5 μm
2.5 cm × 6 mm
807166 TSKgel® QAE-2SW HPLC Column ammonium ion, quaternary phase
5 μm
25 cm × 4.6 mm