TSKgel® Cation Exchange HPLC Columns

Ion exchange chromatography separates molecules based on differences in the net charge of the molecules. In cation exchange, the analyte is positively charged (a cation), while the chromatographic material is negatively charged (an anion). Ion exchange is usually used for protein purification, but it may be used for purification of oligonucleotides, peptides, or other charged molecules. For interaction to occur, the protein of interest must have a charge opposite to that of the functional group of the sorbent particle. For example, immunoglobulins, which generally have a net positive charge, will bind well to cation exchangers because they possess negatively-charged functional groups. Because this interaction is ionic, binding must take place under low ionic strength conditions. Elution is achieved by increasing the ionic strength of the mobile phase to reduce ionic attractions, or by changing the pH of the mobile phase to alter the ionization state of the protein.

TSKgel columns are highly efficient for sample purification with excellent recovery. Anion- and cation-exchangers are available on porous polymer-based and silica-based particles. Proteins and nucleic acids can be analyzed faster on a TSKgel non-porous resin column, although STAT and NPR columns have lower capacity than large pore-size-polymer particles. TSKgel BioAssist S columns are constructed from PEEK (polyether ether ketone).

TSKgel® CM-5PW Columns (1000 Å pore size)

Product #

Description

Phase

Particle Size

Length × I.D.

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813069 TSKgel® CM-5PW Guardgel Kit carboxymethyl phase
10 μm
2.5 cm × 6 mm
813068 TSKgel® CM-5PW HPLC Column carboxymethyl phase
10 μm
7.5 cm × 7.5 mm

TSKgel® SP-5PW Columns (1000 Å pore size)

Product #

Description

Phase

Particle Size

Length × I.D.

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808807 TSKgel® SP-5PW Guardgel Kit sulfonic acid phase
20 μm
1 cm × 8 mm
807211 TSKgel® SP-5PW Guardgel Kit sulfonic acid phase
20 μm
2.5 cm × 6 mm
816093 TSKgel® SP-5PW Guardgel Kit sulfonic acid phase
20 μm
3.5 cm × 10 mm
814017 TSKgel® SP-5PW HPLC Column sulfonic acid phase
13 μm
15 cm × 20 mm
813062 TSKgel® SP-5PW HPLC Column sulfonic acid phase
10 μm
5 cm × 5 mm
808803 TSKgel® SP-5PW HPLC Column sulfonic acid phase
10 μm
7.5 cm × 8 mm
807161 TSKgel® SP-5PW HPLC Column sulfonic acid phase
10 μm
7.5 cm × 7.5 mm
818758 TSKgel® SP-5PW HPLC Column sulfonic acid phase
10 μm
7.5 cm × 2 mm
807575 TSKgel® SP-5PW HPLC Column sulfonic acid phase
13 μm
15 cm × 21.5 mm

TSKgel® BioAssist® S Columns (1300 Å pore size)

Product #

Description

Phase

Particle Size

Length × I.D.

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819686 TSKgel® BioAssist® S HPLC Column sulfonic acid phase
7 μm
5 cm × 4.6 mm
821411 TSKgel® BioAssist® S HPLC Column sulfonic acid phase
13 μm
10 cm × 10 mm

TSKgel® NPR Columns (non-porous)

Product #

Description

Phase

Particle Size

Length × I.D.

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813076 TSKgel® SP-NPR HPLC Column sulfonic acid phase
2.5 μm
3.5 cm × 4.6 mm

TSKgel® STAT Columns (non-porous)

Product #

Description

Phase

Particle Size

Length × I.D.

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821966 TSKgel® CM-STAT HPLC Column carboxymethyl phase
7 μm
10 cm × 4.6 mm
821965 TSKgel® CM-STAT HPLC Column carboxymethyl phase
10 μm
3.5 cm × 3 mm
821964 TSKgel® SP-STAT HPLC Column sulfonic acid phase
7 μm
10 cm × 4.6 mm
821963 TSKgel® SP-STAT HPLC Column sulfonic acid phase
10 μm
3.5 cm × 3 mm

Silica-based TSKgel® Cation Exchange Columns

Product #

Description

Phase

Particle Size

Length × I.D.

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807167 TSKgel® CM-2SW HPLC Column carboxymethyl phase
5 μm
25 cm × 4.6 mm
807162 TSKgel® CM-3SW HPLC Column carboxymethyl phase
10 μm
7.5 cm × 7.5 mm
807650 TSKgel® CM-SW Guardgel Kit carboxymethyl phase
10 μm
2.5 cm × 6 mm
807165 TSKgel® SP-2SW HPLC Column sulfonic acid phase
5 μm
25 cm × 4.6 mm
807644 TSKgel® SP-SW Guardgel Kit sulfonic acid phase
5 μm
2.5 cm × 6 mm