Ascentis® Express F5, 2.7μm HPLC Column

The pentafluorophenylpropyl (PFP) stationary phase of Ascentis® Express F5 provides a stable, reversed-phase packing with electron-deficient phenyl rings due to the presence of electronegative fluorines. In addition to forming p-p and mildly steric interactions, F5 phases also retain compounds by polar interactions. As a result of having both polar and non-polar character, F5 phases can show dual-mode retention behavior, sometimes producing a “U-shaped” retention as a function of acetonitrile content of the mobile phase, with retention increasing at both low and high concentrations of ACN (reversed-phase and HILIC retention modes). Ascentis Express F5 can be used for basic, acidic, or neutral compounds with alternate selectivity from C18.

Learn More About Fluorinated Stationary Phases
Alternative Retention and Selectivity Using Fluorinated Stationary Phases
Fast and Accurate LC-MS Analysis of Vitamin D Metabolites Using Ascentis Express F5 HPLC Columns

F5, 2.7μm Columns

Product #

Description

Particle Size

Length × I.D.

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53569-U Ascentis® Express F5, 2.7 Micron HPLC Column 2.7 μm
10 cm × 2.1 mm
53578-U Ascentis® Express F5, 2.7 Micron HPLC Column 2.7 μm
10 cm × 3.0 mm
53590-U Ascentis® Express F5, 2.7 Micron HPLC Column 2.7 μm
10 cm × 4.6 mm
53571-U Ascentis® Express F5, 2.7 Micron HPLC Column 2.7 μm
15 cm × 2.1 mm
53579-U Ascentis® Express F5, 2.7 Micron HPLC Column 2.7 μm
15 cm × 3.0 mm
53591-U Ascentis® Express F5, 2.7 Micron HPLC Column 2.7 μm
15 cm × 4.6 mm
53592-U Ascentis® Express F5, 2.7 Micron HPLC Column 2.7 μm
2 cm × 2.1 mm
53566-U Ascentis® Express F5, 2.7 Micron HPLC Column 2.7 μm
3 cm × 2.1 mm
53574-U Ascentis® Express F5, 2.7 Micron HPLC Column 2.7 μm
3 cm × 3.0 mm
53581-U Ascentis® Express F5, 2.7 Micron HPLC Column 2.7 μm
3 cm × 4.6 mm
53567-U Ascentis® Express F5, 2.7 Micron HPLC Column 2.7 μm
5 cm × 2.1 mm
53576-U Ascentis® Express F5, 2.7 Micron HPLC Column 2.7 μm
5 cm × 3.0 mm
53583-U Ascentis® Express F5, 2.7 Micron HPLC Column 2.7 μm
5 cm × 4.6 mm
53568-U Ascentis® Express F5, 2.7 Micron HPLC Column 2.7 μm
7.5 cm × 2.1 mm
53577-U Ascentis® Express F5, 2.7 Micron HPLC Column 2.7 μm
7.5 cm × 3.0 mm
53584-U Ascentis® Express F5, 2.7 Micron HPLC Column 2.7 μm
7.5 cm × 4.6 mm

F5, 2.7μm Guard Cartridges and Holder

Product #

Description

Particle Size

Length × I.D.

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53594-U Ascentis® Express F5, 2.7 Micron Guard Cartridge 2.7 μm
5 mm × 2.1 mm
53597-U Ascentis® Express F5, 2.7 Micron Guard Cartridge 2.7 μm
5 mm × 3.0 mm
53599-U Ascentis® Express F5, 2.7 Micron Guard Cartridge 2.7 μm
5 mm × 4.6 mm
53500-U Ascentis® Express Guard Cartridge Holder