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TSKgel® Polymer-based Reversed-Phase HPLC Columns

To separate large biomolecules, such as peptides and proteins by reversed phase (RP), the pore size of the support particle is an important consideration. Because retention can be strong and the mobile phase contains an organic modifier, RP is usually avoided with proteins that are needed to be recovered in their active forms.

Tosoh Bioscience offers reversed-phase columns for biopolymer separations packed with silica or methacrylate particles. The hydrophilic backbone of polymer-based reversed phase columns improves recovery and reduces secondary interactions. The polymethacrylate particles are also stable from pH 1 to 12 and do not swell in organic solvents; they can withstand rigorous cleaning with either acid or base. TSKgel Octadecyl-NPR columns are packed with non-porous methacrylate particles of uniform 2.5 micron size for high efficiency separations and fast analyses of peptides and proteins. The non-porous particle structure limits product isolation to sub-microgram loads.

TSKgel® Octadecyl-NPR Columns (non-porous)

Product #

Description

Phase

Particle Size

Length × I.D.

Add to Cart

814005 TSKgel® Octadecyl-NPR HPLC Column C18 (octadecyl) phase
2.5 μm
3.5 cm × 4.6 mm

TSKgel® Phenyl-5PW RP Columns (1000 Å pores)

Product #

Description

Phase

Particle Size

Length × I.D.

Add to Cart

842159 TSKgel® Phenyl-5PW RP Guard Cartridge phenyl phase
10 μm
1 cm × 2 mm
819007 TSKgel® Phenyl-5PW RP Guard Cartridge phenyl phase
10 μm
1.5 cm × 3.2 mm
816260 TSKgel® Phenyl-5PW RP HPLC Column phenyl phase
13 μm
15 cm × 21.5 mm
818756 TSKgel® Phenyl-5PW RP HPLC Column phenyl phase
10 μm
7.5 cm × 2 mm
808043 TSKgel® Phenyl-5PW RP HPLC Column phenyl phase
10 μm
7.5 cm × 4.6 mm

TSKgel® Octadecyl-4PW Columns (500 Å pores)

Product #

Description

Phase

Particle Size

Length × I.D.

Add to Cart

842160 TSKgel® Octadecyl-4PW Guard Cartridge C18 (octadecyl) phase
7 μm
1 cm × 2 mm
819008 TSKgel® Octadecyl-4PW Guard Cartridge C18 (octadecyl) phase
7 μm
1.5 cm × 3.2 mm
816257 TSKgel® Octadecyl-4PW HPLC Column C18 (octadecyl) phase
13 μm
15 cm × 21.5 mm
818755 TSKgel® Octadecyl-4PW HPLC Column C18 (octadecyl) phase
7 μm
15 cm × 2 mm
813351 TSKgel® Octadecyl-4PW HPLC Column C18 (octadecyl) phase
7 μm
15 cm × 4.6 mm

TSKgel® Octadecyl-2PW Columns (125 Å pores)

Product #

Description

Phase

Particle Size

Length × I.D.

Add to Cart

842161 TSKgel® Octadecyl-2PW Guard Cartridge C18 (octadecyl) phase
5 μm
1 cm × 2 mm
817502 TSKgel® Octadecyl-2PW Guard Column C18 (octadecyl) phase
5 μm
1 cm × 4.6 mm
817503 TSKgel® Octadecyl-2PW Guard Column C18 (octadecyl) phase
5 μm
1 cm × 6 mm
817500 TSKgel® Octadecyl-2PW HPLC Column C18 (octadecyl) phase
5 μm
15 cm × 4.6 mm
817501 TSKgel® Octadecyl-2PW HPLC Column C18 (octadecyl) phase
5 μm
15 cm × 6 mm
818754 TSKgel® Octadecyl-2PW HPLC Column C18 (octadecyl) phase
5 μm
15 cm × 2 mm