Sugars and Saccharides

Simple sugars using hydrophilic interaction chromatography (HILIC): Chromatographic analysis of simple sugars can be challenging because these compounds are highly polar, uncharged, and lack a chromophore. The preferred mode of HPLC separation is HILIC because it provides the advantage of retaining these highly polar compounds that are otherwise hard to separate.
  • Polymeric columns bonded with aminopropyl groups offer improved stability and MS compatibility. Our apHera NH2 column is based on covalently bonded polyamine to co-polymer which offers stability from pH 2-12, mechanical and chemical strength, and high column efficiency.
  • For faster analysis on any HPLC system, turn to Ascentis Express. These columns, based on Fused-Core technology, offer the performance of sub-2 μm particles but exhibit the backpressure of 3 μm particles. Several phases (HILIC, OH5, and F5) are available that are suitable for operation in the HILIC mode.
  • The Ascentis Express F5 phase is also available in a more traditional 5 μm particle. The Fused-Core technology results in increased efficiency, whereas the 5 μm particles exhibit limited backpressure, allowing longer column lengths than that possible with the standard 2.7 μm Ascentis Express particles.

Simple sugars using ion exclusion HPLC: Ion exclusion HPLC is another method employed for the determination of simple sugars. Our SUPELCOGEL resin-based ion exchange HPLC columns contain spherical particles of sulfonated polystyrene/divinylbenzene, each with specific counter ions. Each counter ion (Ca, H, Pb, K, or Ag) imparts a unique selectivity for the analysis of sugars or organic acids. When the sample matrix is a beverage, limited sample preparation is required. For example, a grape juice cocktail can simply be passed through a 0.45 μm syringe filter prior to analysis.

Saccharides using ion exclusion HPLC: Because the chemical and physical properties of various saccharides are similar, they are more difficult to analyze than many other classes of compounds. HPLC is typically the mode of analysis, relying on differences in conformation, configuration, and bonding mode. However, no single HPLC column or method is capable of separating all saccharides. For this reason, we offer a selection of SUPELCOGEL ion exclusion columns which are specifically prepared for various saccharide analyses. One of these, SUPELCOGEL C-611, contains two divalent cations rather than one, and provides a different selectivity compared to the other SUPELCOGEL columns.

Enzymatic food kits: Enzymatic methods for food analysis are highly specific and offer considerable time and cost savings over other methods, especially from the sample preparation standpoint. Sigma-Aldrich offers a wide variety of convenient kits and reagents for rapid and reliable enzymatic food analysis.