Peptides

The tryptic digest of proteins can be used to make smaller peptides, which can be chromatographed using reverse phase HPLC columns. This is possible because trypsin will cleave a protein the same way each time. Peptide maps can then be used to assist with identification of the source protein.

Technical Article:
Peptide Mapping with Ascentis Express Peptide ES-C18
Separation of Proteins and Peptides on Discovery BIO Wide Pore (300 Å) C18, C8, and C5 Phases
High-Efficiency Ion-Exchange Separations of Proteins and Peptides on Discovery BIO PolyMA