TSKgel® SuperSW, SuperSW mAb and UltraSW Aggregate Columns

TSKgel SuperSW columns, introduced in 1997, represent the third generation of high performance Gel Filtration columns that have become synonymous with analyzing protein molecular weights in the emerging field of biotechnology. TSKgel SuperSW-type columns contain smaller particles than TSKgel SWxl-type columns; 4 micron versus 5 micron. In addition, the column internal diameter has been reduced from 7.8mm ID to 4.6mm ID to provide higher sensitivity in sample-limited cases and to cut down on solvent use; in addition to 4.6 mm ID, TSKgel SuperSW3000 columns are also available in 2 mm and 1 mm ID column formats. It is important to employ an HPLC system that is optimized with regards to extra-column bandbroadening to take full advantage of the high column efficiency that can be obtained on TSKgel SuperSW columns. The TSKgel SW mAb product line consist of three specialized columns designed for the separation and analysis of monoclonal antibodies (mAb). While mAbs can be analyzed using many different modes of HPLC, size exclusion is best to determine aggregation, dimer, and fragmentation, making it the best method for heterogeneity studies. The TSKgel SW mAb columns include:
  • TSKgel SuperSW mAb HR column is best suitaed for high resolution analysis of mAb monomer and dimer
  • TSKgel SuperSW mAb HTP column is optimized for high throughput analysis of mAb monomer and dimer (UHPLC compatible)
  • TSKgel UltraSW Aggregate column provides the best solution for the analysis of mAb aggregates.Compared to competitive columns, these stainless steel, silica-based TSKgel columns offer reduced lot-to-lot variation, long column life, reduction of unspecified adsorption, and superior recovery of aggregates.
The TSKgel SW mAb columns utilize a unique pore-controlled technology, which produces a shallow calibration curve in the molecular weight region of a typical monoclonal antibody. The calibration curve for the TSKgel SuperSW mAb HR column is similar to that of the TSKgel G3000SWxl column and has a shallower slope than the TSKgel UltraSW Aggregate column around the molecular weight range of gamma-globulin. This shallow calibration curve produces high resolution separations. The TSKgel UltraSW Aggregrate calibration curve shows a separation range up to around 2 million Da, which implies better resolution of aggregate/multimer of a monoclonal antibody.

Product #

Description

Phase

Particle Size

Length × I.D.

Add to Cart

818762 TSKgel® SuperSW Guard Column diol phase
4 μm
3.5 cm × 4.6 mm
822857 TSKgel® SuperSW mAb HR Guard Column diol phase
4 μm
4 cm × 6 mm
822854 TSKgel® SuperSW mAb HR HPLC Column diol phase
4 μm
30 m × 7.8 mm
822858 TSKgel® SuperSW mAb HTP Guard Column diol phase
4 μm
2 cm × 3 mm
822855 TSKgel® SuperSW mAb HTP HPLC Column diol phase
4 μm
15 cm × 4.6 mm
818674 TSKgel® SuperSW2000 HPLC Column diol phase
4 μm
30 cm × 4.6 mm
818675 TSKgel® SuperSW3000 HPLC Column diol phase
4 μm
30 cm × 4.6 mm
821485 TSKgel® SuperSW3000 HPLC Column diol phase
4 μm
30 cm × 2 mm
821845 TSKgel® SuperSW3000 HPLC Column diol phase
4 μm
30 cm × 1 mm
822859 TSKgel® UltraSW Aggregate Guard Column diol phase
3 μm
4 cm × 6 mm
822856 TSKgel® UltraSW Aggregate HPLC Column diol phase
3 μm
30 cm × 7.8 mm