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Protein-based Chiral HPLC Columns

Hermansson described the use of natural proteins immobilized onto a silica support for chiral separations in 1983 (1). Proteins contain a large number of chiral centers of one configuration, and many other sites that contribute to the general retention process. We offer three CSPs with proteins as the chiral selectors, CHIRALPAK AGP (a1-acid glycoprotein), CHIRALPAK CBH (cellobiohydrolase) and CHIRALPAK HSA (human serum albumin). All are manufactured by DAICEL Corporation. They are typically used in reversed-phase mode, and perform a wide variety of chiral separations. CHIRALPAK HSA is also used for drug-binding studies. Solutes are retained by three types of interactions: ionic (for charged solutes), hydrophobic and hydrogen bonding. The relative contribution of the different forces to solute retention depends on the nature of
the analyte.
  • CHIRALPAK AGP: Extremely broad applicability. First choice when developing methods on protein-CSPs.
  • CHIRALPAK HSA: Analytes are typically very hydrophilic acids.
  • CHIRALPAK CBH: Analytes are typically very hydrophilic amines and amino alcohols.

Protein-based CSP features and application areas:
  • Direct reversed-phase resolution of chiral molecules
  • Stable in a variety of organic modifiers
  • Available in analytical and semi-preparative sizes
  • CHIRALPAK HSA is also used for drug-binding studies

(1) Hermansson, J. Direct liquid chromatographic resolution of racemic drugs using a1-acid glycoprotein as the chiral stationary phase. J. Chromatogr. A, 1983, 269, 71-80.