CHIROBIOTIC kits contain 5 ��m V2, T, R and TAG in either 10 cm or 25 cm x 4.6 mm I.D. dimensions, and the CHIROBIOTIC guidebook. The kits are an economical means to create a column-screening library.
Method development on CHIROBIOTIC follows a simple strategy that tests polar ionic, polar organic, reversed-phase and normal phase modes. The polar ionic mode has proven most successful. Following are the recommended CHIROBIOTIC screening and optimization protocols:
Polar Ionic Mode (ionizable analytes): Screening: (100:0.1:0.1, v/v/v) CH3OH/acetic acid/triethylamine, Optimization: Change acid-base ratio, change the type of acid or base, add a volatile salt (test different ammonium salts)
Polar Organic Mode (neutral analytes): Screening: 100% Ethanol, Optimization: Use other polar organic solvents or blends
Reversed-Phase (all molecular types): Screening: (30:70) CH3CN/20mM ammonium acetate, pH 4.0, Optimization: Change the % and type of organic modifier, adjust pH, buffer type and ionic strength
Normal Phase Mode (neutral analytes): Screening: (30:70) Ethanol/heptane, Optimization: Increase % of polar modifier, change both solvents
We also offer CYCLOBOND kits to round out your chiral method development, as well as protein-based, copper ligand exchange (CLC) and polycyclic amine polymer (P-CAP) CSPs.
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