CYCLOBOND II AC columns offer phases bonded to gamma-cyclodextrin with the hydroxyl groups in the 2 and 3 position acetylated. As a result, the mouth of the cavity has available a hydrogen acceptor site suitable to interact with a hydrogen donor, such as an amine attached to at least two fused rings or larger. An example would be 1- or 2- substituted napthylethylamine. Applications include steroids and sterols, depending on where the hydroxyl groups are positioned.