This new derivative of CYCLOBOND technology has dinitrophenyl functionality bonded through an ether linkage to the secondary hydroxyl positions of the beta-cyclodextrin. In this arrangement, a π electron sharing system is established with analytes having π systems (e.g., aromatic rings, carbonyl) in the stereogenic environment. Use of the ether linkage to anchor this π acidic dinitrophenyl ring results in a very stable system even under strong reversed phase conditions. The acidity of this group is further enhanced with the introduction of the trifluoromethyl group in the aromatic ring. Except in a few cases this stationary phase demonstrates enhanced selectivity over the conventional CYCLOBOND stationary phase. In a number of cases selectivity was demonstrated only on the CYCLOBOND I 2000 DNP phase, for instance 1,1′-bi-2-naphthol. While the CYCLOBOND I 2000 DNP demonstrates selectivity in all three mobile phase conditions, reversed phase, normal phase and polar organic phase, the reversed phase conditions yield the greatest number of separations and highest selectivity. It has also been observed that buffers can have a dramatic effect on selectivity, especially when employing ammonium phosphate.
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