Ascentis® Express HILIC, 2.7μm HPLC Columns

Suitable for L3 per USP

HILIC chromatography is a variation of normal-phase chromatography with the distinction that one of the major components of the mobile phase is water. The high volatility of the mobile phase makes HILIC LC-MS friendly, and allows for increased MS sensitivity. Many classes of polar compounds can be retained on the Ascentis® Express HILIC column; including, polar neutrals, polar acids, and polar and non-polar basic amines. The HILIC column provides orthogonal selectivity to the Ascentis Express C18, with the elution typically the opposite of that for C18. The non-polar compounds elute early and the highly polar compounds have greater retention and selectivity.

Learn More About HILIC Chromatography
Articles:
Metabolites - A Serious Challenge for LC/MS Separations and Usual Detection Techniques
Metabolomic Profiling of Neurospora crassa Fungi Using HILIC and Reversed-Phase LC-MS
Ascentis Express HILIC Guard Cartridge Holder

HILIC, 2.7μm Columns

Product #

Description

Particle Size

Length × I.D.

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53939-U Ascentis® Express HILIC, 2.7 Micron HPLC Column 2.7 μm
10 cm × 2.1 mm
53979-U Ascentis® Express HILIC, 2.7 Micron HPLC Column 2.7 μm
10 cm × 4.6 mm
53946-U Ascentis® Express HILIC, 2.7 Micron HPLC Column 2.7 μm
15 cm × 2.1 mm
53981-U Ascentis® Express HILIC, 2.7 Micron HPLC Column 2.7 μm
15 cm × 4.6 mm
53933-U Ascentis® Express HILIC, 2.7 Micron HPLC Column 2.7 μm
3 cm × 2.1 mm
53964-U Ascentis® Express HILIC, 2.7 Micron HPLC Column 2.7 μm
3 cm × 3.0 mm
53934-U Ascentis® Express HILIC, 2.7 Micron HPLC Column 2.7 μm
5 cm × 2.1 mm
53975-U Ascentis® Express HILIC, 2.7 Micron HPLC Column 2.7 μm
5 cm × 4.6 mm

HILIC, 2.7μm Guard Cartridges and Holder

Product #

Description

Particle Size

Length × I.D.

Add to Cart

53520-U Ascentis® Express HILIC, 2.7 Micron Guard Cartridge 2.7 μm
5 mm × 2.1 mm
53521-U Ascentis® Express HILIC, 2.7 Micron Guard Cartridge 2.7 μm
5 mm × 3.0 mm
53523-U Ascentis® Express HILIC, 2.7 Micron Guard Cartridge 2.7 μm
5 mm × 4.6 mm
53500-U Ascentis® Express Guard Cartridge Holder