HILIC chromatography is a variation of normal-phase chromatography with the distinction that one of the major components of the mobile phase is water. The high volatility of the mobile phase makes HILIC LC-MS friendly, and allows for increased MS sensitivity. Many classes of polar compounds can be retained on the Ascentis® Express HILIC column; including, polar neutrals, polar acids, and polar and non-polar basic amines. The HILIC column provides orthogonal selectivity to the Ascentis Express C18, with the elution typically the opposite of that for C18. The non-polar compounds elute early and the highly polar compounds have greater retention and selectivity.
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