HybridSPE®-Phospholipid Technology

Effective Removal of Phospholipids and Proteins for Accurate and Reproducible LC-MS Analyses

Key Features
  • Simple methodology, analogous to standard protein precipitation
  • Available in both 96-well plate and cartridge formats (see product list below)
  • Unique chemistry for excellent recovery of both hydrophobic and hydrophilic analytes
  • Part of our bioanalytical LC-MS workflow solution
Reduce Overall Cost per Analysis, Increase Throughput and Improve LC-MS Data Consistency

Product overview video

Visit the HybridSPE home page to learn more about:
New! HybridSPE-PLus plates; the basics of HybridSPE methodology; impact of phospholipids on LC-MS results; and the use of HybridSPE technology for phospholipid enrichment
HybridSPE-Phospholipid Ultra Cartridges
HybridSPE-PLus 96-Well Plate

HybridSPE® Plates

96-well plates are an excellent choice for higher-throughput analyses. We offer HybridSPE-PLus plates, suitable for sample volumes between 100 and 300 μL; as well as our Small Volume HybridSPE-Phospholipid plates, suitable for sample volumes between 20 and 40 μL, in a 96-well plate format. Plates are sold individually as well as in bulk packs of 20 plates.

For analysts who are new to the technology, we also offer a HybridSPE-PLus Plate Essentials Kit (Part number 52818-U) that includes one 96-well HybridSPE-PLus plate, one plate cap mat, one sealing film and a collection plate.

Product #

Description

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52794-U HybridSPE®-Phospholipid Small Volume 96-well Plate, bed wt. 15 mg, volume 0.8 mL, pk of 1
52798-U HybridSPE®-Phospholipid Small Volume 96-well Plate, bed wt. 15 mg, volume 0.8 mL, pk of 20
575659-U HybridSPE®-PLus 96-Well Plate bed wt. 50 mg, volume 2 mL, pk of 1 ea
575673-U HybridSPE®-PLus 96-Well Plate bed wt. 50 mg, volume 2 mL, pk of 20 ea
52818-U HybridSPE®-PLus 96-Well Plate Essentials Kit 96-Well Essentials Kit, pk of 1
55263-U 96-Well Protein Precipitation Filter Plate pk of 1

HybridSPE® Plate Accessories

Product #

Description

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Z721581 EZ-Pierce films Excel Scientific, EZP-100, non-sterile
Z717266 Nunc® 96 DeepWell plate, non-treated maximum volume 2 mL, non-sterile, natural polypropylene wells round bottom
57192-U PlatePrep 96-well Vacuum Manifold Manifold only
575680-U 96 Round Well Pierceable Cap Mats for use with HybridSPE-PLus 96-well plates, pk of 50 ×

HybridSPE® Cartridges

Cartridges allow for easy processing of just one or a few samples at a time. Like our 96-well plate products, the 1 mL HybridSPE-Phospholipid Ultra cartridge is capable of removing both proteins and phospholipids in an online format. If an offline protein precipitation is preferred, the 1 mL or 6 mL HybridSPE-Phospholipid cartridges, which do not contain a protein precipitation filter, can be used for phospholipid removal following a separate offline protein precipitation (using a product such as Cat. No. 55263-U, 96-well protein precipitation filter plate to remove precipitated proteins from the sample first).

Product #

Description

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55261-U HybridSPE®-Phospholipid Cartridge, bed wt. 30 mg, volume 1 mL, pk of 100
55276-U HybridSPE®-Phospholipid Cartridge, bed wt. 30 mg, volume 1 mL, pk of 200, polypropylene material (hardware), PE frit (20 μm)
55267-U HybridSPE®-Phospholipid Cartridge, bed wt. 500 mg, volume 6 mL, pk of 30
55269-U HybridSPE®-Phospholipid Ultra cartridge, bed wt. 30 mg, volume 1 mL, pk of 100

HybridSPE® Cartridge Accessories

Product #

Description

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57059 Disposable Liners for Visiprep DL Manifolds (included with 57044 and 57265) PTFE, pk of 100
57044 Visiprep SPE Vacuum Manifold DL (Disposable Liner), 12-port model
57265 Visiprep SPE Vacuum Manifold DL (Disposable Liner), 24-port model
57030-U Visiprep SPE Vacuum Manifold standard, 12-port model
57250-U Visiprep SPE Vacuum Manifold standard, 24-port model

Precipitation Solvents, Blends, Additives

Product #

Description

Add to Cart

251275 Citric acid ACS reagent, ≥99.5%

Related Equipment

In addition to the related equipment listed below, we also offer a variety of KNF Laboport® Vacuum Pumps. Please inquire for details.

Product #

Description

Add to Cart

Z654760 IKA® VORTEX 3 vortex shaker, 115V, 1/cs
Z654779 IKA® VORTEX 3 vortex shaker, 230V, 1/cs
57161-U SPE Manifold Gauge/Bleed Valve Remote In-Line Design
57120-U SPE Vacuum Pump Trap Kit