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Products for Amino Acid Hydrolysis

Hydrolysis is typically achieved by acid conditions. A standard procedure is hydrolysis with 6 M hydrochloric acid (24 hours, 110°C). These standard procedure is a compromise between time requirement and temperature. Sensitive amino acids (especially tryptophan and cysteine) will be partially destroyed. Gas phase hydrolysis and addition of other acids (e.g. propionic acid, TFA, methanesulfonic acid) can be used to shorten the hydrolysis time and improve the yield of sensitive amino acids.

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P3303 Endoproteinase Asp-N from Pseudomonas fragi mutant strain suitable for protein sequencing, lyophilized powder
P6181 Endoproteinase Glu-C from Staphylococcus aureus V8 suitable for protein sequencing, lyophilized powder
P2289 Endoproteinase Lys-C from Lysobacter enzymogenes lyophilized powder
45167 Endoproteinase Pro-C, recombinant from E. coli ~3 U/mg
77249 Heptafluorobutyric acid ≥99.0% (GC)
84429 Hydrochloric acid solution ~6 M in H2O, for amino acid analysis
64280 Methanesulfonic acid ≥99.0%, ReagentPlus®
402907 Propionic acid ACS reagent, ≥99.5%
91709 Trifluoroacetic acid solution BioReagent, suitable for protein sequencing, ~25% in H2O