Ion Pair Reagents

The use of ion-pairing reagents as mobile phase additives allows the separation of ionic and highly polar substances on reversed phase HPLC columns. The purity of mobile phase additives is of utmost importance to their successful application.
Sigma-Aldrich offers an outstanding range of tailor-made reagents for anionic (quaternary ammonium and phosphonium salts) and cationic (alkanesulfonates) determination . All mobile phase additives are subject to rigorous testing with special emphasis on the requirements of modern reversed phase HPLC:
  • Filter test
  • UV-absorption test
  • Test for the absence of redox traces with a cyclic voltammogram
  • HPLC gradient grade test

Acidic Ion Pair Reagents (Anionic)

Acidic samples can be separated with straight-chain alkyl quaternary ammonium salts or alkyl amines. The pH of the mobile phase needs to be ajdusted to 7.5 and diluted with the HPLC solvents (acetonitrile/water or methanol/water) to 5mM.

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30518 Decamethonium bromide for ion pair chromatography, ≥99.0% (AT)
44239 Dodecyltrimethylammonium bromide for ion pair chromatography, ≥98.5% (AT)
44243 Dodecyltrimethylammonium hydrogen sulfate for ion pair chromatography, ≥99.0% (T)
49540 Heptafluorobutyric acid solution 0.5 M in H2O, for ion pair chromatography
52371 Hexadecyltrimethylammonium bisulfate for ion pair chromatography, ≥99.0% (T)
52367 Hexadecyltrimethylammonium bromide for ion pair chromatography
87208 Myristyltrimethylammonium bromide for ion pair chromatography, ≥99.0% (AT)
86853 Tetrabutylammonium bisulfate for ion pair chromatography, ≥99.0%
86857 Tetrabutylammonium bromide for ion pair chromatography, ≥99.0%
86852 Tetrabutylammonium chloride for ion pair chromatography, ≥99.0%
86862 Tetrabutylammonium chloride solution for ion pair chromatography, concentrate, ampule
86854 Tetrabutylammonium hydroxide solution ~40% in water, for ion chromatography
86903 Tetrabutylammonium iodide for ion pair chromatography, ≥99.0%
86899 Tetrabutylammonium phosphate monobasic solution for ion pair chromatography, concentrate, ampule
86608 Tetraethylammonium bromide for ion pair chromatography, ≥99.0%
86626 Tetraethylammonium hydrogen sulfate for ion pair chromatography, ≥99.0%
87296 Tetraheptylammonium bromide for ion pair chromatography, ≥99.0% (AT)
87297 Tetrahexylammonium bromide for ion pair chromatography, ≥99.0% (AT)
87299 Tetrahexylammonium hydrogensulfate for ion pair chromatography, ≥99.0% (T)
87578 Tetrakis(decyl)ammonium bromide for ion pair chromatography, ≥99.0% (AT)
87724 Tetramethylammonium bisulfate for ion pair chromatography, ≥99.0% (T)
87708 Tetramethylammonium bromide for ion pair chromatography, ≥99.0% (AT)
02799 Tetramethylammonium sulfate for ion pair chromatography, ≥99.0% (T)
87996 Tetraoctylammonium bromide for ion pair chromatography, ≥99.0% (AT)
87997 Tetrapentylammonium bromide for ion pair chromatography, ≥99.0% (AT)
88106 Tetrapropylammonium bisulfate for ion pair chromatography, ≥99.0%
88103 Tetrapropylammonium bromide for ion pair chromatography, ≥99.0% (AT)
87215 Trimethyltetradecylammonium hydrogen sulfate for ion pair chromatography, ≥99.0% (T)

Basic Ion Pair Reagents (Cationic)

Basic samples can be separated by addition of a straight-chain alkyl sulphonic acid to the mobile phase. The pH of the mobile phase is adjusted to 3-4 and diluted with the HPLC solvents (acetonitrile/water or methanol/water) to 5mM.

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19022 Sodium 1-butanesulfonate for ion pair chromatography, ≥99.0% (T)
30631 Sodium 1-decanesulfonate for ion pair chromatography, ≥99.0%
71443 Sodium decyl sulfate for ion pair chromatography, ≥99.0% (T)
71726 Sodium dodecyl sulfate for ion pair chromatography, ≥99.0%
02374 Sodium 1,2-ethanedisulfonate for ion pair chromatography, ≥99.0% (T)
51832 Sodium 1-heptanesulfonate monohydrate for ion pair chromatography, ≥99.0% (T)
51834 Sodium 1-heptanesulfonate solution for ion pair chromatography, concentrate, ampule
52862 Sodium 1-hexanesulfonate monohydrate for ion pair chromatography, ≥99.0% (T)
52864 Sodium 1-hexanesulfonate solution for ion pair chromatography, concentrate, ampule
70289 Sodium 2-naphthalenesulfonate for ion pair chromatography, ≥99.0% (HPLC)
74316 Sodium 1-nonanesulfonate for ion pair chromatography, ≥99.0% (T)
74882 Sodium 1-octanesulfonate monohydrate for ion pair chromatography, ≥99.0% (T)
74886 Sodium 1-octanesulfonate solution for ion pair chromatography, concentrate, ampule
75073 Sodium octyl sulfate for ion pair chromatography, ≥99.0% (T)
76952 Sodium 1-pentanesulfonate monohydrate for ion pair chromatography, ≥99.0% (T)
76954 Sodium 1-pentanesulfonate solution for ion pair chromatography, concentrate, ampule
81806 Sodium 1-propanesulfonate monohydrate for ion pair chromatography, ≥99.0% (T)
87191 Sodium 1-tetradecanesulfonate for ion pair chromatography, ≥99.0% (T)