SPME for HPLC and Bioanalysis: Interface, Fibers, Probes, and Accessories

Traditional solid phase microextraction (SPME) fibers were developed with common GC type phases, which were primarily designed for extraction of volatile or semi-volatile compounds using thermal desorption as the means of introducing sample into the chromatographic column. However, SPME is now seeing growth in its applications in the HPLC/LC-MS realm, especially in bioanalysis.

New SPME Fiber Probes for HPLC and Bioanalytical Applications (57281-U)

We now offer SPME fibers with a new coating designed specifically for extraction of polar and nonpolar analytes using solvent desorption for LC applications. The newly developed fiber probes contain C18 silica particles embedded in a proprietary, non-swelling, biocompatible polymer. The benefit of this design enables minimized binding of macromolecules such as proteins and phospholipids, but allows extraction of most smaller analytes of interest. The fiber core is made from a flexible metal alloy with shape memory properties and a diameter larger than typical SPME fibers. The larger diameter enables the fiber to be used both as the plunger and fiber core, thus simplifying the device construction and reducing the cost of the probe. The fiber is also sealed into a hypodermic needle with an attached hub to allow movement of the fiber for exposure and retraction into the needle. This design enables the fiber assembly to be pierced into a catheter shunt, vial septa, or the fiber can be exposed for direct immersion into a liquid sample.

Traditional HPLC-Compatible Fibers and Interface

The interface allows mobile phase to contact the SPME fiber, remove the adsorbed analytes, and deliver them to the column for LC separation. It consists of a 6-port injection valve and a desorption chamber; it can be easily installed and removed. The fiber assemblies recommended for HPLC use are crimped (rather than attached with epoxy). All have a 24-gauge needle and are suitable for autosampler-HPLC use.