Discovery^® HS F5

Discovery™ HS F5 Exhibits "U-Shape" Retention Profile
Under certain mobile phase conditions and with certain analytes, certain polar phases, like Discovery™ HS F5, can exhibit both reversed-phase and normal-phase behavior. At low percent organic, retention decreases with increasing percent organic following reversed-phase behavior. However, at higher percent organic, retention increases with increasing percent organic following normal-phase behavior. The result is a "U-shape" retention profile for these compounds. If your compounds exhibit this U-shape profile, use it to your advantage to:

• Improve LC-MS detection by using higher % organic mobile phase
• Use mobile phase concentration to alter selectivity at high % organic

HS F5 bonded fluoro (pentafluorophenyl) columns provide separations that are different from C18 columns. Unique selectivity plus the ability to transfer a C18 method make this an ideal complimentary reversed-phase for screening.