TSKgel® Gel Filtration Columns for Proteins and other Biopolymers

TSKgel SW, SWxl and SuperSW (SW-type) columns are silica-based. A hydrophilic diol-type bonded phase shields the silica surface from interacting with protein samples. Compared to competitive HPLC columns, SW-type columns contain more pore volume per unit column volume, which results in higher MW selectivity or better resolution. Three different pore sizes are available:* 125Å pore size for analysis of small proteins and peptides* 250Å pore size for most protein samples and monoclonal antibody (mAb) monomer and dimer* 300Å pore size for mAb aggregates and multimers * 450Å pore size for very large proteins and nucleic acidsParticle sizes for the four SW-type column lines are 4 micron (SuperSW), 5-8 micron (SWxl), 10-13 micron (SW), and 3-4 micron (SW mAb).

TSKgel UP-SW3000, the new 2 micron UHPLC column with 25 nm pore size, is the latest addition to the renowned TSKgel SW series. TSKgel UP-SW3000 represents the fifth generation of high performance gel filtration columns. They feature the same pore size as the well-established TSKgel G3000SWXL and facilitate method transfer from conventional gel filtration to UHPLC technology. The 15 cm column is used to shorten analysis time while maintaining resolution. The 30 cm column delivers dramatically increased resolution between fragments, monomers, and aggregates.

TSKgel® SuperSW, SuperSW mAb and UltraSW Aggregate Columns

TSKgel SuperSW columns, introduced in 1997, represent the third generation of high performance Gel Filtration columns that have become synonymous with analyzing protein molecular weights in the emerging field of biotechnology. TSKgel SuperSW-type columns contain smaller particles than TSKgel SWxl-type columns; 4 micron versus 5 micron. In addition, the column internal diameter has been reduced from 7.8mm ID to 4.6mm ID to provide higher sensitivity in sample-limited cases and to cut down on solvent use; in addition to 4.6 mm ID, TSKgel SuperSW3000 columns are also available in 2 mm and 1 mm ID column formats. It is important to employ an HPLC system that is optimized with regards to extra-column bandbroadening to take full advantage of the high column efficiency that can be obtained on TSKgel SuperSW columns. The TSKgel SW mAb product line consist of three specialized columns designed for the separation and analysis of monoclonal antibodies (mAb). While mAbs can be analyzed using many different modes of HPLC, size exclusion is best to determine aggregation, dimer, and fragmentation, making it the best method for heterogeneity studies. The TSKgel SW mAb columns include:
  • TSKgel SuperSW mAb HR column is best suitaed for high resolution analysis of mAb monomer and dimer
  • TSKgel SuperSW mAb HTP column is optimized for high throughput analysis of mAb monomer and dimer (UHPLC compatible)
  • TSKgel UltraSW Aggregate column provides the best solution for the analysis of mAb aggregates.Compared to competitive columns, these stainless steel, silica-based TSKgel columns offer reduced lot-to-lot variation, long column life, reduction of unspecified adsorption, and superior recovery of aggregates.
The TSKgel SW mAb columns utilize a unique pore-controlled technology, which produces a shallow calibration curve in the molecular weight region of a typical monoclonal antibody. The calibration curve for the TSKgel SuperSW mAb HR column is similar to that of the TSKgel G3000SWxl column and has a shallower slope than the TSKgel UltraSW Aggregate column around the molecular weight range of gamma-globulin. This shallow calibration curve produces high resolution separations. The TSKgel UltraSW Aggregrate calibration curve shows a separation range up to around 2 million Da, which implies better resolution of aggregate/multimer of a monoclonal antibody.

Product #

Description

Phase

Particle Size

Length × I.D.

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818762 TSKgel® SuperSW Guard Column diol phase
4 μm
3.5 cm × 4.6 mm
822857 TSKgel® SuperSW mAb HR Guard Column diol phase
4 μm
4 cm × 6 mm
822854 TSKgel® SuperSW mAb HR HPLC Column diol phase
4 μm
30 m × 7.8 mm
822858 TSKgel® SuperSW mAb HTP Guard Column diol phase
4 μm
2 cm × 3 mm
822855 TSKgel® SuperSW mAb HTP HPLC Column diol phase
4 μm
15 cm × 4.6 mm
818674 TSKgel® SuperSW2000 HPLC Column diol phase
4 μm
30 cm × 4.6 mm
818675 TSKgel® SuperSW3000 HPLC Column diol phase
4 μm
30 cm × 4.6 mm
821485 TSKgel® SuperSW3000 HPLC Column diol phase
4 μm
30 cm × 2 mm
821845 TSKgel® SuperSW3000 HPLC Column diol phase
4 μm
30 cm × 1 mm
822859 TSKgel® UltraSW Aggregate Guard Column diol phase
3 μm
4 cm × 6 mm
822856 TSKgel® UltraSW Aggregate HPLC Column diol phase
3 μm
30 cm × 7.8 mm

TSKgel® UP-SW3000 Columns

TSKgel UP-SW3000, the new 2 micron UHPLC column with 25 nm pore size, is the latest addition to the renowned TSKgel SW series. TSKgel UP-SW3000 represents the fifth generation of high performance gel filtration columns. They feature the same pore size as the well-established TSKgel G3000SWXL and facilitate method transfer from conventional gel filtration to UHPLC technology. The 15 cm column is used to shorten analysis time while maintaining resolution. The 30 cm column delivers dramatically increased resolution between fragments, monomers, and aggregates.

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Product #

Description

Phase

Particle Size

Length × I.D.

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80023451 TSKgel® UP-SW Direct Connect Guard Column for P/N 80023448/9 diol phase
2 μm
2 cm × 4.6 mm
80023450 TSKgel® UP-SW Guard Column for P/N 8002348/9 diol phase
2 μm
15 cm × 4.6 mm
80023448 TSKgel® UP-SW3000 HPLC Column diol phase
2 μm
30 cm × 4.6 mm
80023449 TSKgel® UP-SW3000 HPLC Column diol phase
2 μm
15 cm × 4.6 mm

TSKgel® SWXL Type and QC-PAK GFC Columns

TSKgel SWxl columns, introduced in 1987, represent the second generation of high performance Gel Filtration columns that have become synonymous with analyzing protein molecular weights in the emerging field of biotechnology. TSKgel SWxl-type columns contain smaller particles than TSKgel SW-type columns; 5 and 8μm versus 10 and 13μm. As the TSKgel SW-type columns, TSKgel SWxl columns feature highly porous silica particles, the surface of which has been shielded from interacting with proteins by derivatization with ligands containing diol functional groups. TSKgel SWxl-type columns distinguish themselves from other silica- or polymer-based high performance size exclusion columns by virtue of their large pore volumes. These columns are commonly used in the quality control of monoclonal antibodies and other biopharma products. TSKgel BIOassist columns are packed in PEEK hardware.

Product #

Description

Phase

Particle Size

Length × I.D.

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808540 TSKgel® G2000SWXL HPLC Column diol phase
5 μm
30 cm × 7.8 mm
808541 TSKgel® G3000SWXL HPLC Column diol phase
5 μm
30 cm × 7.8 mm
808542 TSKgel® G4000SWXL HPLC Column diol phase
8 μm
30 cm × 7.8 mm
816215 TSKgel® QC-PAK GFC 200 HPLC Column diol phase
5 μm
15 cm × 7.8 mm
816049 TSKgel® QC-PAK GFC 300 HPLC Column diol phase
5 μm
15 cm × 7.8 mm
820027 TSKgel® SWXL Columns diol phase
5 μm
30 cm × 7.8 mm
820026 TSKgel® SWXL Columns diol phase
5 μm
30 cm × 7.8 mm
820025 TSKgel® SWXL Columns diol phase
8 μm
30 cm × 7.8 mm
818008 TSKgel® SWXL Columns diol phase
7 μm
4 cm × 6 mm
808544 TSKgel® SWXL Top-Off Gel diol phase
5 μm
 
808543 TSKgel® SWXL Type Guard Column diol phase
7 μm
4 cm × 6 mm

TSKgel® SW Type Columns

TSKgel SW columns, introduced in 1977, were the first of a long line of high performance Gel Filtration columns that have become synonymous with analyzing protein molecular weights in the emerging field of biotechnology. TSKgel SW-type columns are based on highly porous silica particles, the surface of which has been shielded from interacting with proteins by derivatization with ligands containing diol functional groups. TSKgel SW-type columns distinguish themselves from other silica- or polymer-based high performance size exclusion columns by virtue of their large pore volumes."

Product #

Description

Phase

Particle Size

Length × I.D.

Add to Cart

805102 TSKgel® G2000SW HPLC Column diol phase
10 μm
60 cm × 7.5 mm
805788 TSKgel® G2000SW HPLC Column diol phase
10 μm
30 cm × 7.5 mm
806727 TSKgel® G2000SW HPLC Column diol phase
13 μm
30 cm × 21.5 mm
805146 TSKgel® G2000SW HPLC Column diol phase
13 μm
60 cm × 21.5 mm
808800 TSKgel® G3000SW HPLC Column diol phase
10 μm
30 cm × 8 mm
805103 TSKgel® G3000SW HPLC Column diol phase
10 μm
60 cm × 7.5 mm
805789 TSKgel® G3000SW HPLC Column diol phase
10 μm
30 cm × 7.5 mm
806728 TSKgel® G3000SW HPLC Column diol phase
13 μm
30 cm × 21.5 mm
805147 TSKgel® G3000SW HPLC Column diol phase
13 μm
60 cm × 21.5 mm
808801 TSKgel® G4000SW HPLC Column diol phase
13 μm
30 cm × 8 mm
805104 TSKgel® G4000SW HPLC Column diol phase
13 μm
60 cm × 7.5 mm
805790 TSKgel® G4000SW HPLC Column diol phase
13 μm
30 cm × 7.5 mm
805148 TSKgel® G4000SW HPLC Column diol phase
17 μm
60 cm × 21.5 mm
806729 TSKgel® G4000SW HPLC Column diol phase
17 μm
30 cm × 21.5 mm
805371 TSKgel® SW Type Guard Column diol phase
10 μm
7.5 cm × 7.5 mm
805758 TSKgel® SW Type Guard Column diol phase
13 μm
7.5 cm × 21.5 mm
808805 TSKgel® SW-Type Guard Column diol phase
10 μm
4 cm × 8 mm