To protect your column from alkaline mobile phases which can dissolve silica (pH >7.0), saturate the mobile phase with silica by passing it through a silica saturator column. To protect your column from acidic mobile phases which can strip bonded phase (pH <2.0), use a C18 saturator column. Each kit consists of 10 g of packing (12 μm spherical particles, 180 m2/g surface area), a 7.5 cm × 4.6 mm column, two frits, and fittings to connect the column to 1/16 in. tubing. The optional plastic funnel is helpful for filling these columns (Cat. No. 20390-U).
;)
