PHRED: Photochemical Reactor Enhanced Detection

A post-column photochemical reactor enhancement detection system (PHRED) can improve analyte detection in the presence of interfering matrix components, or when the
analytes are present at low concentrations. Tamoxifen, a nonsteroid estrogen antagonist widely used in treating breast cancer, and its major metabolite, 4-hydroxytamoxifen, are literally undetected at 260nm. With post-column photolysis, however, detection is highly reliable. Use of in-line photochemical derivatization to screen for aflatoxins is simple, reliable, reproducible, and sensitive. A PHRED system can be used in simultaneous HPLC analysis of aflatoxins, zearalenone, and ochratoxin A.