GC Derivatizing Agents

Why is chemical derivatization needed?

Gas chromatography, a technique for separation of volatile compounds which are thermally stable, is unfortunately not always easily to apply for compounds of biomedical and environmental interest, particularly for those of high molecular weight or containing polar functional groups. These groups are difficult to analyze by GC either because they are not sufficiently volatile, tail badly, are too strongly attracted to the stationary phase, thermally unstable or even decomposed.

Chemical derivatization prior to analysis is generally done to:

increase the volatility and decrease the polarity of compounds;
reduce thermal degradation of samples by increasing their thermal stability;
increase detector response by incorporating functional groups which lead to higher detector signals, e.g. CF₃ groups for electron capture detectors;
improve separation and reduce tailing
enlarge substrate spectrum

What kind of Derivatizing Reagents exist ?

Common derivatization methods can be classified into 4 groups depending on the type of reaction applied:

Silylation
Acylation
Alkylation
Esterification

To view the list of derivatizing agents for all methods, please click on the method above.

Due to our broad experience in production and analysis of such derivatizing reagents and especially of silylating agents we can assure that you only get reliable, clearly specified and customer-focused products.

Silylating Reagents
Catalog No.	Product	CAS No.	Description
05940	Allyldimethylchlorosilane	4028-23-3	purum; ≥97.0% (GC)
09324	3-Aminopropyltriethoxysilane	919-30-2	purum; ~99% (GC)
09326	3-Aminopropyltrimethoxysilane	13822-56-5	purum; ≥97.0% (NT)
14660	1,3-Bis(chloromethyl)tetramethyl-disilazane	14579-91-0	purum; ~97% (GC)
14700	Bis(diethylamino)dimethylsilane	4669-59-4	purum; ~97% (GC)
14755	Bis(dimethylamino)dimethylsilane	3768-58-9	purum; ~97% (GC)
15242	N,O-Bis(trimethylsilyl)acetamide, 10x1ml	10416-59-8	purum; ~97% (GC)
15269	N,O-Bis(trimethylsilyl)acetamide, for GC	10416-59-8	purum p.a. for GC; ≥97.0% (GC)
15256	N,O-Bis(trimethylsilyl)acetamide with 5% TMCS, for GC		for GC
15236	N,O-Bis(trimethylsilyl)carbamate	35342-88-2	purum; ≥98.0% (T)
15247	N,N-Bis(trimethylsilyl)formamide	15500-60-4	pract.; ~95% (GC)
15235	N,N-Bis(trimethylsilyl)methylamine	920-68-3	purum; ≥97.0% (GC)
15249	Bis(trimethylsilyl)sulfate	18306-29-1	purum; ≥98.0% (T)
15198	N,O-Bis(trimethylsilyl)trifluoroacetamide, for GC, 10x1ml	25561-30-2	puriss. p.a. for GC; ≥99.0% (GC)
15222	N,O-Bis(trimethylsilyl)trifluoroacetamide, for GC	25561-30-2	puriss. p.a. for GC; ≥99.0% (GC)
15243	N,O-Bis(trimethylsilyl)trifluoroacetamide	25561-30-2	purum; ≥98.0% (GC)
15238	N,O-Bis(trimethylsilyl)trifluoroacetamide with 1% TMCS	25561-30-2	for GC
15239	N,O-Bis(trimethylsilyl)trifluoroacetamide with 1% TMCS 10x1ml	25561-30-2	for GC
15209	N,O-Bis(trimethylsilyl)trifluoroacetamide with 10% TMCS	95596-72-8	for GC
15248	N,N'-Bis(trimethylsilyl)urea	18297-63-7	purum; ≥98.0% (N)
17615	(Bromomethyl)dimethylchlorosilane	16532-02-8	purum; ~98% (GC)
19903	Butyldimethylchlorosilane	1000-50-6	purum; ≥97.0% (GC)
19904	tert-Butyldimethylchlorosilane Solution, ~1 M in THF	18162-48-6	purum; ~1 M in tetrahydrofuran
19920	1-(tert-Butyldimethylsilyl)imidazole	54925-64-3	purum; ~97% (GC)
19915	N-tert-Butyldimethylsilyl-N-methyltrifluoroacetamide	77377-52-7	purum; ≥97.0% (GC)
19918	N-tert-Butyldime.silyl-N-me.trifluoroacetamide + 1% TBDMSCl	77377-52-7	purum
19938	tert-Butyldiphenylchlorosilane	58479-61-1	purum; ≥97.0% (GC)
25117	(Chloromethyl)dimethylchlorosilane	1719-57-9	purum; ~97% (GC)
26230	(3-Chloropropyl)dimethylchlorosilane	10605-40-0	pract.; ≥95% (GC)
26250	(3-Chloropropyl)trimethoxysilane	2530-87-2	purum; ~97% (GC)
28470	(2-Cyanoethyl)trichlorosilane	1071-22-3	purum; ≥98.0% (GC)
28560	3-Cyanopropyltrichlorosilane	1071-27-8	purum; ≥98.0% (GC)
28558	3-Cyanopropyltriethoxysilane	1067-47-6	purum; ≥96% (GC)
32575	N,N-Diethyltrimethylsilylamine	996-50-9	purum; ~97% (GC)
15226	(+)-Diisopropyl O,O'-bis(trimethylsilyl)-L-tartrate	130678-42-1	ChiraSelect; ~99% (GC, sum of enantiomers)
15223	(-)-Diisopropyl O,O'-bis(trimethylsilyl)-D-tartrate	197013-45-9	ChiraSelect; ~99% (GC, sum of enantiomers)
39900	Dimethylchlorosilane	1066-35-9	BioChemika purum; ~96% (GC)
40140	Dimethyldichlorosilane	75-78-5	BioChemika puriss.; ≥99,5% (GC)
40925	Dimethyl(3,3,4,4,5,5,6,6,6-nonafluorohe-xyl)chlorosil.(2 ml)	119386-82-2	purum; ≥97.0% (GC)
41663	1,3-Dimethyl-1,1,3,3-tetraphenyldi-silazane	7453-26-1	purum; ≥98.0% (NT)
41720	N,N-Dimethyltrimethylsilylamine	2083-91-2	purum; ~97% (GC)
43300	Diphenylsilanediol	947-42-2	purum; ≥97.0% (CH)
43340	1,3-Diphenyl-1,1,3,3-tetramethyl disila-zane	3449-26-1	purum; ≥97.0% (GC)
43930	1,3-Divinyl-1,1,3,3-tetramethyldisilazane	7691-02-3	purum; ~97% (GC)
44230	Dodecyltrichlorosilane	4484-72-4	purum; ~97% (GC)
44235	Dodecyltrichlorosilane	4484-72-4	puriss.; ≥99.0% (GC)
44237	Dodecyltriethoxysilane	18536-91-9	pract.; ≥95% (GC)
92756	Ethyl trimethylsilylacetate	4071-88-9	purum; ~97% (GC)
50040	(3-Glycidyloxypropyl)trimethoxysilane	2530-83-8	purum; ≥97.0% (GC)
52614	2,2,4,4,6,6-Hexamethylcyclotrisilazane	1009-93-4	purum; ≥97.0% (GC)
52619	Hexamethyldisilazane	999-97-3	puriss.; ≥99.0% (GC)
52630	Hexamethyldisiloxane	107-46-0	puriss.; ~99% (GC)
52643	Hexamethyldisilthiane	3385-94-2	purum
58810	(3-Isocyanatopropyl)triethoxysilane	24801-88-5	pract.; ≥95% (GC)
63797	(3-Mercaptopropyl)triethoxysilane	14814-09-6	techn.; ≥80% (GC)
63800	(3-Mercaptopropyl)trimethoxysilane	4420-74-0	purum; ≥97.0% (GC)
68210	Methyl-octyldichlorosilane	14799-93-0	purum; ≥98.0% (GC)
92758	(Methylthio)trimethylsilane	3908-55-2	purum; ≥97.0% (GC)
69450	Methyltrichlorosilane	75-79-6	puriss.; ≥99.0% (AT)
69480	N-Methyl-N-trimethylsilylacetamide	7449-74-3	purum; ≥97.0% (GC)
69484	N-Methyl-N-trimethylsilylheptafluorobutyramide	53296-64-3	pract.; ≥90% (GC)
69479	N-Methyl-N-trimethylsilyltrifluoroacet-amide, for GC	24589-78-4	puriss. for GC; ~99% (GC)
69482	N-Methyl-N-trimethylsilyltrifluoroacetamide	24589-78-4	purum; ≥97.0% (GC)
74762	Octadecyltrichlorosilane	112-04-9	pract.; ~90% (GC)
74891	Octaphenylcyclotetrasiloxane	546-56-5	purum; ≥98.0% (CH)
75085	Octyltrichlorosilane	5283-66-9	purum; ≥98.0% (GC)
76750	(Pentafluorophenyl)dimethylchlorosilane	20082-71-7	purum; ≥97.0% (GC)
85431	Silylating mixture Fluka I according to Sweeley for GC		for GC; ready to use solution in pyridine (10 vol parts)
85434	Silylating mixture Fluka I acc. to Sweeley (10x1 ml)		for GC; ready to use solution in pyridine (10 vol parts); ampoule
85432	Silylating mixture Fluka II acc. to Horning for GC		for GC
85435	Silylating mixture Fluka II acc. to Horning (10x1 ml)		for GC; ampoule
85433	Silylating mixture Fluka III for GC		for GC
85436	Silylating mixture Fluka III for GC(10x1 ml)		for GC; ampoule
87830	1,1,3,3-Tetramethyldisilazane	15933-59-2	purum; ≥97.0% (GC)
90383	Triethylchlorosilane	994-30-9	purum; ≥97.0% (GC)
90385	Triethylchlorosilane Solution,~1 M in THF	994-30-9	purum; ~1 M in tetrahydrofuran
91743	Triethylsilyl trifluoromethanesulfonate	79271-56-0	purum; ≥98.0% (T)
92090	Triisopropylchlorosilane	13154-24-0	purum; ~97% (GC)
91746	Triisopropylsilyl trifluoromethanesulfonate	80522-42-5	purum; ≥98.0% (T)
92337	Trimethylbromosilane, stab.	2857-97-8	purum; ≥97.0% (AT); stab. with silver wool
92360	Trimethylchlorosilane	75-77-4	puriss.; ≥99.0% (GC)
92741	4-Trimethylsiloxy-3-penten-2-one	13257-81-3	purum; ≥97.0% (GC)
92745	N-(Trimethylsilyl)acetamide	13435-12-6	pract.; ~90% (GC)
92753	Trimethylsilyl azide	4648-54-8	purum; ~97% (GC)
12700	Trimethylsilyl benzenesulfonate	17882-06-3	purum; ≥98.0% (T)
39860	Trimethylsilyl N,N-dimethylcarbamate	32115-55-2	purum; ~97% (GC)
92751	1-(Trimethylsilyl)imidazole	18156-74-6	purum; ≥98.0% (T)
92718	1-(Trimethylsilyl)imidazole/Pyridine1/4 (v/v), for GC	8077-35-8	for GC
64296	Trimethylsilyl methanesulfonate	10090-05-8	purum
77245	Trimethylsilyl nonafluoro-1-butane-sulfonate	68734-62-3	purum; ≥98.0% (T)
91741	Trimethylsilyl trifluoromethanesulfonate	27607-77-8	purum; ≥98.0% (T)
93133	Triphenylsilylamine	4215-80-9	purum; ~97% (NT)

Back to top

Acylating Reagents
Catalog No.	Product	CAS No.	Description
19667	Butylboronic acid, for GC	4426-47-5	BioChemika for GC; ~98% (T)
77251	Heptafluorobutyric anhydride, 10x1 ml	336-59-4	puriss.; ≥99.0% (GC)
77253	Heptafluorobutyric anhydride	336-59-4	puriss.; ≥99.0% (GC)
77254	Heptafluorobutyryl chloride	375-16-6	pract.; ≥98.0% (GC)
02379	2,3,4,5,6-Pentafluorobenzoic anhydride	15989-99-8	purum; ≥98.0% (GC)
77290	Pentafluoropropionic acid	422-64-0	purum; ≥97.0% (GC)
77292	Pentafluoropropionic anhydride	356-42-3	purum; ~98% (GC)
80903	Pivalic anhydride	1538-75-6	pract.; ≥97.0% (GC)
80910	Pivaloyl chloride	3282-30-2	purum; ≥98.0% (GC)
91719	Trifluoroacetic anhydride, derivatization reagent	407-25-0	BioChemika puriss. p.a. derivatization reagent for GC; ≥99.0% (GC)
91720	Trifluoroacetic anhydride	407-25-0	purum; ≥98.0% (GC)

Back to top

Alkylating and Esterifying Reagents
Catalog No.	Product	CAS No.	Description
15716	Boron trifluoride (~10% (~1.3 M) inMethanol)	373-57-9	puriss. for esterification of fatty acids for GC purposes
16460	4-Bromobenzyl bromide	589-15-1	purum; ≥98.0% (GC)
17910	alpha-Bromo-2,3,4,5,6-pentafluorotoluene	1765-40-8	puriss.; ≥99.0% (GC)
18790	alpha-Bromo-p-xylene	104-81-4	purum; ≥97.0% (GC)
40263	N,N-Dimethylformamide di-tert-butylacetal	36805-97-7	pract.; ≥90% (GC)
40252	N,N-Dimethylformamide diethyl acetal	1188-33-6	pract. for esterification of fatty acids; ~95% (GC)
40271	N,N-Dimethylformamide dimethyl acetal	4637-24-5	pract.; ≥95% (GC)
40680	N,N-Dimethylhydrazine (asym.) anhydrous	57-14-7	purum; ≥98.0% (GC)
52517	1,1,1,3,3,3-Hexafluoro-2-propanol	920-66-1	puriss. p.a. for GC; ≥99,8% (GC)
17934	Hydrogen chloride 1.25 M in Ethanol	123864-74-4	puriss. p.a. for GC; ~1.25 M (T)
17935	Hydrogen chloride 1.25 M in Methanol	132228-87-6	puriss. p.a. for GC; ~1.25 M (T)
17933	Hydrogen chloride 1.25 in 2-Propanol		puriss. p.a. for GC; ~1.25 M (T)
64954	2-Methoxy-1,3-dioxolane	19693-75-5	purum; ≥98.0% (GC)
66638	O-Methyl-N,N'-diisopropylisourea	54648-79-2	BioChemika purum; ≥97.0% (GC)
77256	Nonafluoro-1-iodobutane, stab.	423-39-2	purum; ≥98.0% (GC); stab. with silver wool
77265	Pentafluoro-iodoethane	354-64-3	purum; ≥97.0% (GC)
76757	2,2,3,3,3-Pentafluoro-1-propanol	422-05-9	purum; ≥97.0% (GC)
79265	Phenyltrimethylammonium hydroxide Sol.,~0.1 M in methanol	1899-02-1	puriss. p.a. for GC; ~0.1 M in methanol
79266	Phenyltrimethylammonium hydroxide Sol.,~0.5 M in methanol	1899-02-1	puriss. p.a. for GC; ~0.5 M in methanol
87285	2,3,5,6-Tetrafluoro-4-(trifluoromethyl)benzyl bromide	76437-40-6	purum; ≥97.0% (GC)
91120	2,2,2-Trichloroethanol	115-20-8	BioChemika pract.; ~95% (GC)
91690	2,2,2-Trifluoroethanol	75-89-8	puriss.; ≥99.0% (GC)
92334	Trimethylboroxine	823-96-1	puriss.; ≥99.0% (GC)
92732	Trimethylsulfonium hydrox. Sol., ~0.25 M in MeOH, 10x1 ml	17287-03-5	purum derivatization reagent for GC; ~0.25 M in methanol

your favorite gene search

Service & Support

Ordering

Corporate

©2011 Sigma-Aldrich Co. LLC. All rights reserved. SIGMA, SAFC, SIGMA-ALDRICH, ALDRICH, and SUPELCO are trademarks of Sigma-Aldrich Co. LLC, registered in the US and other countries. FLUKA is a trademark of Sigma-Aldrich GmbH, registered in the US and other countries. Sigma brand products are sold through Sigma-Aldrich, Inc. Purchaser must determine the suitability of the product(s) for their particular use. Additional terms and conditions may apply. Site Use Terms | Privacy

Product Directory

Custom Product

Services Offered

Custom Capabilities

Email this page to a friend

GC Derivatizing Agents

your favorite gene search

Service & Support

Ordering

Corporate