All chromatographic methods -with the exception of TLC- use columns for the separation process. Column chromatography has found it's place in many laboratories for preparative purposes as well as for reaction control in organic syntheses. The importance of column chromatography is mainly due to following factors:
- Simple packing procedure
- Low operating pressure
- Low expense for instrumentation
The following table gives you an overview of all supports and adsorbents for column chromatography offered by Fluka. When choosing a column packing, you have to consider the properties of substances to be separated-including impurities or components of no interest-and then select the proper adsorbent. As a rule of thumb you can consider:
- For lipophilic substances: aluminum oxide, silica, acetylated cellulose, polyamide
- For hydrophilic substances: cellulose, kieselgur, polyamide
In addition to this, it's important to consider possible reactions between the compounds and the adsorbent or eluent. In practice, silica gel is the preferred stationary phase for separation of organics.
| Cat.No |
Name |
Package size |
| 05074 |
Agarose 2 B for chromatography |
25 ml |
| 05076 |
Agarose 4 B for chromatography |
25 ml |
| 05078 |
Agarose 6 B for chromatography |
25 ml |
| 06290 |
Aluminum oxide Fluka, type 5016 A basic |
1 kg, 5 kg, 25 kg |
| 06300 |
Aluminum oxide Fluka, type 507 C neutral, 100-125 mesh |
1 kg, 5 kg, 25 kg |
| 06310 |
Aluminum oxide Fluka, type 506 C weakly acidic |
1kg |
| 06320 |
Aluminum oxide Fluka, type 504 C acidic |
1 kg, 2.5 kg |
| 09297 |
Aminopropyl silica gel, for preparative chromatography |
50 g, 250 g |
| 09298 |
Aminopropyl silica gel, for preparative chromatography |
100g |
| 11365 |
Avicel® PH-101, ~50 mm |
250 g, 1kg |
| 15685 |
Boric acid Gel, for column chromatography, 0.1-0.4 mm |
5 ml, 25 ml |
| 21900 |
Carboxymethylcellulose Sodium salt; low viscosity |
100 g, 250 g, 1kg |
| 21902 |
Carboxymethylcellulose Sodium salt; medium viscosity |
100 g, 250 g, 1kg |
| 21903 |
Carboxymethylcellulose Sodium salt; high viscosity |
100 g, 250 g, 1kg |
| 21904 |
Carboxymethylcellulose Sodium salt; ultra high viscosity |
100 g, 250 g, 1kg |
| 21901 |
Carboxymethylcellulose Sodium salt; ultra low viscosity |
100 g, 250 g, 1kg |
| 22182 |
Cellulose powder, spruce, for column chromatography, acid washed |
250 g, 1kg |
| 22181 |
Cellulose powder from spruce, for column chromatography |
250 g, 1kg |
| 22184 |
Cellulose powder, for column chromatography, acid washed |
250 g, 1kg |
| 22183 |
Cellulose powder, for column chromatography |
250 g, 1kg |
| 02563 |
Cellulose acetate SAC 20, acetylated |
250g |
| 22192 |
Cellulose acetate phthalate |
100 g, 500 g |
| 22200 |
Cellulose triacetate |
50 g, 250 g, 1kg |
| 22503 |
Chelite P, Na+-form, 25-40 mesh |
100 g, 500 g |
| 26960 |
Cholestyramine resin, for column chromatography |
50 g, 250 g |
| 27560 |
CM-Dextran Sodium salt |
5 g, 25 g |
|
|
CM Dextranomer C-25-120 Synonym Carboxymethyl-dextran |
5 g. 25 g, 100 g |
|
|
CM Dextranomer C-50-120 Synonym Carboxymethyl-dextran |
5 g. 25 g, 100 g |
| 27563 |
CM-Sepharose® CL-6B, for chromatography |
50 ml, 250 ml |
| 27565 |
CM-Sepharose® 'Fast Flow', for chromatography |
100 ml, 500 ml |
| 30477 |
DEAE-Cellulose, 100-200 mm |
25 g, 100 g |
| 30461 |
DEAE-Dextran hydrochloride, for molecular biology |
25 g, 100 g |
| 30462 |
DEAE-Dextran hydrochloride |
25 g, 100 g |
| 30473 |
DEAE-Sepharose® CL-6B, for chromatography |
25 ml, 100 ml, 500 ml |
| 30474 |
DEAE-Sepharose® 'Fast Flow', for chromatography* |
50 ml, 250 ml |
| 31399 |
Dextrangel N 25 for column chromatography |
5 g, 25 g, 100 g |
| 44785 |
Ecteola-23 Cellulose |
100 g, 500 g |
| 29227 |
FlashTubeTM 2002 |
1ST |
| 29229 |
FlashTubeTM 2008 |
1ST |
| 03286 |
Florisil® acc. to ISO 9377-2/H53, for Water Analysis; further details |
100 g, 500 g |
| 46381 |
Florisil® for Chromatography, 16-30 mesh |
100 g, 500 g |
| 46384 |
Florisil® for Chromatography, 30-60 mesh |
100 g, 500 g |
| 46385 |
Florisil® for Chromatography, 60-100 mesh |
100 g, 500 g |
| 46386 |
Florisil® for Chromatography, 100-200 mesh |
100 g, 500 g |
| 46382 |
Florisil® PR, for pesticide residue analysis 60-100 mesh |
100 g, 500 g |
| 55496 |
Hydroxylapatite 'high resolution' |
10 g, 50 g |
| 55497 |
Hydroxylapatite 'fast flow' |
10 g, 50 g |
| 02395 |
Polyamide 6, for column chromatography |
250 g |
| 83332 |
QAE-Sepharose® 'Fast Flow', for chromatography |
100 ml, 500 ml |
| 84936 |
Sephacryl® S-200, for chromatography |
100 ml, 500 ml |
| 84937 |
Sephacryl® S-300, for chromatography |
100 ml, 500 ml |
| 84939 |
Sephacryl® S-300-HR, for chromatography |
100 ml, 500 ml |
| 84941 |
Sephadex® G-10 for chromatography, 40-120 mm |
25 g, 100 g, 500 g |
| 84942 |
Sephadex® G-25 fine for chromatography, 20-80 mm |
25 g, 100 g, 500 g |
| 84943 |
Sephadex® G-25 medium for chromatography, 50-150 mm |
10 g, 50 g, 250 g |
| 84944 |
Sephadex® G-25 coarse for chromatography, 100-300 mm |
10 g, 50 g, 250 g |
| 84946 |
Sephadex® G-50 superfine for chromatography, 20-50 mm |
10 g, 50 g, 250 g |
| 84947 |
Sephadex® G-50 fine for chromatography, 20-80 mm |
10 g, 50 g, 250 g |
| 84948 |
Sephadex® G-50 medium for chromatography, 50-150 mm |
10 g, 50 g, 250 g |
| 84949 |
Sephadex® G-75 for chromatography, 40-120 mm |
10 g, 50 g, 250 g |
| 84934 |
Sephadex® G-75-50 superfine, for chromatography |
10 g, 50 g, 250 g |
| 84951 |
Sephadex® G-100 for chromatography, 40-120 mm |
10 g, 50 g, 250 g |
| 84961 |
Sephadex® G-200 superfine, for chromatography |
10 g, 25 g, 100 g |
| 84954 |
Sephadex® CM C-25 for chromatography, 40-120 mm |
10 g, 50 g, 250 g |
| 84955 |
Sephadex® CM C-50 for chromatography, 40-120 mm |
10 g, 50 g, 250 g |
| 84956 |
Sephadex® DEAE A-25 for chromatography, 40-120 mm |
10 g, 50 g, 250 g |
| 84957 |
Sephadex® DEAE A-50 for chromatography, 40-120 mm |
10 g, 50 g, 250 g |
| 84952 |
Sephadex® LH-20 for chromatography, 25-100 mm |
25 g, 100 g, 500 g |
| 84962 |
Sepharose® 4B for chromatography |
100 ml, 250 ml, 1l |
| 84964 |
Sepharose® 6B forchromatography |
250 ml, 1l |
| 84963 |
Sepharose® CL-4B forchromatography |
250 ml, 1l |
| 84965 |
Sepharose® CL-6B forchromatography |
250 ml, 1l |
| 84967 |
Sepharose® 6B epoxy-activated for chromatography |
5 g, 10 g |
| 85636 |
S Sepharose® 'Fast Flow', for chromatography |
100 ml, 500 ml |
| 85328 |
Silica-Alox |
1kg |
| 60734 |
Silica gel 40 for column chromatography 0.015-0.035 mm |
100 g, 500 g |
| 60735 |
Silica gel 40 for column chromatography 0.063-0.2 mm |
250 g, 1kg |
| 60736 |
Silica gel 40 for column chromatography 0.2-0.5 mm |
250 g, 1kg |
| 60750 |
Silica gel 40 for preparative column chromatography 0.04-0.063 mm |
100 g, 500 g |
| 60737 |
Silica gel 60 for production column chromatography 0.04-0.063 mm |
500 g, 2.5kg |
| 60738 |
Silica gel 60 for column chromatography 0.035-0.070 mm |
250 g, , 25 kg, 1kg |
| 60739 |
Silica gel 60 for column chromatography <0.063 mm |
250 g, 1 kg |
| 60740 |
Silica gel 60 for column chromatography 0.063-0.2 mm |
100 g, 500 g |
| 60741 |
Silica gel 60 for column chromatography 0.063-0.2 mm |
250 g, 1 kg, 25 kg |
| 60742 |
Silica gel 60 for column chromatography 0.2-0.5 mm |
250 g, 1 kg, 25 kg |
| 60751 |
Silica gel 60, for for preparative column chromatography <0.04 mm |
100 g, 500 g |
| 60752 |
Silica gel 60 for preparative column chromatography 0.040-0.063 mm |
500 g, 1 kg, 25 kg |
| 00223 |
Silica gel 60, for column chromatography, 20-40 mm |
250g |
| 60743 |
Silica gel 60 F254 for column chromatography. 0.063-0.2 mm |
250 g, 1 kg |
| 60744 |
Silica gel 100 for column chromatography 0.015-0.035 mm |
100 g, 500 g |
| 60745 |
Silica gel 100 for column chromatography 0.063-0.2 mm |
250 g, 1 kg |
| 60746 |
Silica gel 100 for column chromatography 0.2-0.5 mm |
250 g, 1 kg |
| 60753 |
Silica gel 100 for or preparative column chromatography 0.04-0.063 mm |
100 g, 500 g |
| 60747 |
Silica gel 100 CN for column chromatography 0.040-0.063 mm |
50 g, 250 g |
| 60748 |
Silica gel 100 CN for column chromatography0.015-0.035 mm |
50 g, 250 g |
| 60755 |
Silica gel 100 C8-Reversed phase, 0.015-0.035 mm |
50 g, 250 g |
| 60755 |
Silica gel 100 C8-Reversed phase, 0.015-0.035 mm |
50 g, 250 g |
| 60759 |
Silica gel 100 C8-Reversed phase, 0.040-0.063 mm |
50 g, 250 g |
| 60754 |
Silica gel 100 C18-Reversed phase for column chromatography |
50 g, 250 g |
| 60756 |
Silica gel 100 C18-Reversed phase for column chromatography |
50 g, 250 g |
| 60757 |
Silica gel 100 C18-Reversed phase for column chromatography |
50 g, 250 g |
| 60758 |
Silica gel 100 C18-Reversed phase for column chromatography |
50 g, 250 g |
| 03563 |
Silica gel E, 0.5-1.0 mm, according ToIxfeld and Buck |
250 g, 1kg |
| 03559 |
Silica gel FIA, 0.071-0.63 mm, acc. to DIN 51791 |
250 g, 1kg |
| 03561 |
Silica gel FIA, 0.071-0.16 mm, acc. to DIN 51791 |
250 g, 1kg |
| |
Silica gel, spherical, click for further information |
|
| 60780 |
Silicic acid Hydrate, powder, particle size 100 mesh |
100 g, 1kg |
| 60780 |
Silicic acid Hydrate, powder, particle size 100 mesh |
250 g |
| 02388 |
Tribenzoylcellulose Suspension, 10-20 mm, ~40% in Methanol/Water |
100 ml, 500 ml |
Spherical Silica Gel
These high value products are mechanically very stable and ensure excellent column packing reproducibility and column performance. The wide variety of pore size from 70 Å to 300 Å allows the application for a broad range of separation processes including separations of peptides and proteins. Convince yourself about improved chromatography results!
- Porous spherical particles
- Narrow pore and particle size distribution
- High mechanical Stability
- High column packing reproducibility
 |
| Figure 1: Particle size distribution of Silica Gel 100 Å |
| |
|
93875 |
95021 |
86981 |
89752 |
| Nominal pore diam. |
Å |
70 |
100 |
200 |
300 |
| Specific surface area |
m2/g |
480 |
270 |
130 |
90 |
| Specific pore volume |
ml/g |
0.8 |
0.9 |
0.9 |
0.9 |
| pH (10% aqueous solution) |
|
6 |
6 |
6 |
6 |
| Res. Water (160°C) |
% |
<2 |
<2 |
<2 |
<2 |
| Fe |
ppm |
30 |
30 |
30 |
30 |
| Pb |
ppm |
1 |
1 |
1 |
1 |
| Particle sizes |
mm |
40 |
40 |
40 |
40 |
Florisil® for Water Analysis acc. EN ISO 9377-2/H53
Hydrocarbons in water have to be determined according guideline EN ISO 9377-2/H53.
The method requires a clean-up step using Florisil® as adsorbent. We responded to many inquiries from customers and developed Florisil® which is approved according EN ISO 9377-2/H53.
Specifications
- Recovery Rate in Mineral Oil: >80.0 %
- Relation Peak Area Stearylstearate Treated/Untreated: <1.0
- Particle Size: 60-100 mesh
Product List
| Prod.No. |
Name |
Pack Size |
| 03286 |
Florisil® acc. to ISO 9377-2/H53 |
100 g, 500 g |
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