Derivatization Reagents

Acylation Derivatization Reagent


Acylation, an alternative to silylation, is the conversion of compounds with active hydrogen's such as –OH, -SH, and –NH into esters, thioesters and amines, respectively. A very popular example of this method is the insertion of perfluoracyl groups into a substance to enable electron capture detection (ECD). Carbonyl groups adjacent to halogenated carbons enhance the response of ECD.

A further benefit of acylation is the formation of fragmentation-directing derivatives for GC/MS analysis. Acylation reagents can be classified into two main groups: fluoro acid anhydrides and fluoracylimidazoles.

Fluorinated anhydride derivatives of alcohols, phenols and amines are both stable and highly volatile. Fluoracylimidazoles react readily with hydroxyl groups and secondary or tertiary amines to form acyl derivatives. The imidazole produced as a by-product is relatively inert.

View Acylation Products in the Online Catalog

Applications for Acylation:

Acetic Anhydride (PDF 91 KB)
Trifluoroacetic Acid (PDF 100 KB)
Perfluoro Acid Anhydrides (PDF 98 KB)