Derivatization Reagents

Silylation Derivatization Reagent

Silylation is the most widely used derivatization technique. Nearly all functional groups which present a problem in gas chromatogaphic separation (hydroxyl, carboxylic acid, amine, thiol, phosphate) can be derivatized by silylation reagents. It involves the replacement of an acidic hydrogen on the compound with an alkylsilyl group, for example, -SiMe3. The derivatives are generally less polar, more volatile and more thermally stable. The introduction of a silyl group(s) can also serve to enhance mass spectrometric properties of derivatives, by producing either more favorable diagnostic fragmentation patterns of use in structure investigations, or characteristic ions of use in trace analyses employing selected ion monitoring and related techniques. 

View Silylation Products in the Online Catalog

Applications for Silylation

BSA (PDF 107 KB)
BSA + TMCS (PDF 108 KB)
BSA + TMCS + TMSI (PDF 109 KB)
BSTFA (PDF 93 KB)
BSTFA + TMCS (PDF 110 KB)
DMDCS (PDF 85 KB)
5% DMDCS in Toluene (PDF 101 KB)
Analytix Issue 2 / 2004 (PDF 392 KB)
HMDS (PDF 91 KB)
HMDS+TMCS (PDF 92 KB)
HMDS+TMCS+Pyridine (PDF 95 KB)
Rejuv-8TM (PDF 83 KB)
Silylation Overview (PDF 716 KB)
N-t-Butyldimethylsilylimidazole (PDF 90 KB)
TMCS (PDF 105 KB)
TMSI (PDF 104 KB)
TMSI+Pyridine (PDF 105 KB)