Food Analysis

Food Analysis

During the last decade, food protection from potentially hazardous residues has become a major topic of public interests. Food regulation authorities in the European Community and Switzerland produced proposals for restrictions of unwanted residues in food. Fluka can contribute to this trend by offering a comprehensive product range of speciality standards designed for the food and beverage industry. The product range includes unique items such as bisphenol-A-diglycidyl ether(BADGE) and bisphenol-F-diglycidyl ether standards (BFDGE) as well as food irradiation- and phytoestrogene standards.

Please take a look at the following topics:

See also:


Ready-to-use Kit for Acrylamide Analysis

Last year the Swedish National Food Administration published its concerns regarding the high concentrations of acrylamide detected in foods processed at high temperatures. This discovery was particularly disturbing since the acrylamide-containing food stuff included products that are consumed on a regular basis at rather large quantities, such as potato chips, French fries, roast potatoes, breakfast cereals and crisp bread.

The most common method for the determination of acrylamide in food is the Grob-Method. This method requires several high quality reagents and standards that are not available from the same supplier. Fluka has combined efforts and is now presenting a ready-to-use kit that makes acrylamide determination faster and easier. The Acrylamide Kit (Cat. No 72615) contains all the necessary standards and solvents for the extraction and accurate quantification of acrylamide as proposed by Grob1.

Your convenient solution: Acrylamide Kit

It was an exciting challenge for us to provide you with a ready-to-use kit for fast and easy determination of acrylamide. The Kit (Cat. No 72615) contains all reagents and standards needed for 12 determinations. The suitability of all 8 reagents was thoroughly tested. The concentrations and specifications of the Acrylamide-, Acrylamide-D3- and Methacrylamide-Standard Solutions were specially tailored for this method which reduces the time required significantly. The kit contains all the instructions required for sample preparation. Table 1 provides an overview of the kit and the reagents, their specifications and pack sizes. Please click here to download the method (168 KB).

Table 1. Composition of Acrylamide Kit (Cat. No 72615)

Component Specification Package Size
Acrylamide Standard Solution 500 ppm in Acetonitrile 5 ml (Certan® vial)
Acrylamide-D3 Standard Solution 500 ppm in Acetonitrile 5 ml (Certan® vial)
Methylacrylamide Standard Solution 500 ppm in Acetonitrile 5 ml (Certan® vial)
Butyramide Standard Solution 25 ppm in Acetonitrile 5 ml (Certan® vial)
1-Propanol puriss.p.a., >99.5 % (GC) 250 ml glass bottle
n-Hexane puriss., >99.0 % (GC) 100 ml glass bottle
Acetonitrile for residue analysis, >99.9 % (GC) 50 ml glass bottle
Oil tested to determine Acrylamide
according to Grob
50 ml glass bottle

Note: Components of Acrylamide Kit (Cat. No 72615). Standard solutions are available as kit only and cannot be ordered individually. All components last for 12 determinations.

Literature References

  1. M. Biedermann, S. Biedermann-Brem, A. Noti and K. Grob; P. Egli and H. Mändli; Mitt. Lebensm. Hyg. 2002, 93, 638-652.

Standards for food analysis: BADGE, BFDGE

Epoxy based resins are widely used for lacquer coatings on the inside of food cans and food storage vessels. BADGE and/or BFDGE are normally added to these coatings. Studies have shown that the hydrochloric media in the edible oil reacts with the epoxy based resins of the coating, resulting in the corresponding HCl-adducts, of which its toxicities are unknown. BADGE and/or BFDGE are added to the coating in order to prevent these reactions by reacting with hydrochloric acid itself.

There has been performed considerable research in Europe to develop and apply methods of analysis for the food analysis standards. In 2000 the European Scientific Committee for Food produced a proposal for a limit of 1 mg/kg for BADGE, BFDGE (and their adducts) as well as hydrolysates in canned food. For performing a reliable analysis, standards of BADGE, BFDGE and its derivatives are needed. Fluka offers 9 unique, carefully synthesized standards including: BADGE, BFDGE, the HCl-adducts and hydrolysates (see Table 2). There is a large target group of people working with these standards, including all analytical chemists within the food industry (control laboratories), can producing companies, governmental laboratories, private analytical service laboratories and university laboratories.

Table 2. Standards for Food Analysis: BADGE, BFDGE

Catalog No. Product Name CAS No. Description Package Size
15136 Bisphenol A bis(3-chloro-2-hydroxypropyl) ether 4809-35-2 purum p.a. qualitative standard; >99.0% (HPLC) 250 mg, 1g
15137 Bisphenol A bis(2,3-dihydroxypropyl)ether 5581-32-8 purum p.a. qualitative standard; >97.0% (HPLC) 250 mg
92427 Bisphenol A (3-chloro-2-hydroxypropyl) (2,3-dihydroxypropyl) ether 227947-06-0 purum p.a. qualitative standard; >98.0% (HPLC) 25 mg, 100 mg
73124 Bisphenol A (3-chloro-2-hydroxypropyl) glycidyl ether 13836-48-1 purum p.a. qualitative standard; ~95% (HPLC) 25 mg, 100 mg
15138 Bisphenol A diglycidyl ether, standardfor quantification 1675-54-3 purum p.a. qualitative standard; >97.0% (GC) 500 mg
73417 Bisphenol A (2,3-dihydroxypropyl) glycidyl ether 76002-91-0 purum p.a. qualitative standard; ~97% (HPLC) 25 mg, 100 mg
15139 Bisphenol F bis(3-chloro-2-hydroxy-propyl) ether - p.a. qualitative standard; mixture of 3 isomers ortho-ortho, ortho-para, para-para; ~95% (HPLC) total assay of the 3 isomers 250 mg
15142 Bisphenol F bis(2,3-dihydroxypropyl)ether 72406-26-9 purum p.a. qualitative standard; mixture of 3 isomers ortho-ortho, ortho-para, para-para; >97.0% (HPLC) total assay of the 3 isomers 250 mg
15144 Bisphenol F diglycidyl ether 2095-03-6 purum p.a. qualitative standard; mixture of 3 isomers ortho-ortho, ortho-para, para-para; ~97% (GC) total assay of the 3 isomers 500 mg


Food Irradiation

Treatment of food-stuff with ionized radiation is used to eliminate microbiological activity. Such food-stuff, with a few exceptions, cannot be sold in certain European countries and need to be declared in many other countries. Irradiation produces certain substances which are absent in untreated food stuff and have been used as indicators of irradiation. Irradiation of fat-containing foodstuff (e.g. chicken meat, hazelnut) produces free radicals, which partially degrade fat (triglycerides). This process produces alkanes, alkenes, aldehydes and alkylcyclobutanones. Irradiation of water-containing albuminous foodstuffs (meat) produces reactive hydroxyradicals, which react with phenylalanine to produce ortho- and meta-tyrosine. Detection of typical radiation byproducts is used to prove treatment with ionized radiation. Fluka offers selected indicator substances which can be used as standards for GC-analysis (Table 3).

Table 2. Standards for the Detection of Food Irradiation

Catalog No. Product Name CAS No. Description Package Size
52206 1,7-Hexadecadiene 125110-62-5 ~ 95% (GC), standard for food analysis 5 mg, 25 mg
87141 1,7-Tetradecadiene 150767-46-7 ~ 95% (GC), standard for food analysis 5 mg, 25 mg
44197 2-Dodecylcyclobutanone 35493-46-0 > 95% (GC), standard for food analysis 5 mg, 25 mg
93851 DL-o-Tyrosine 2370-61-8 ~ 98% (NT), standard for food analysis 100 mg
93853 DL-m-Tyrosine 775-06-4 > 98.0% (NT), standard for food analysis 100 mg


Phytoestrogens

It is well established, that plants contain substances with estrogenic effects, so called phytoestrogens. These substances have been found in numerous plant food products, e.g. from carrots, peas, lentil, rice, cabbage and soy. They are structurally similar to the mammalian estrogen or estradiol, and show estrogenic properties. However, their estrogenic activity is generally much less than that of human estrogens (estrogenic activity ranges from 1/500 to 1/1000 of the activity of oestradiol). Hence phytoestrogens can act as anti-oestrogenic agents by blocking the oestrogen receptors and exerting a much weaker oestrogenic effect compared with the hormone. As a consequence it has been suggested that they might partly suppress or inhibit normal oestrogenic activity in oestrogen-responsive tissues such as breast tissue and may reduce the risk of breast cancer. Fluka offers a large number of selected pure flavones, flavonones and isoflavones for the examination of phytoestrogenic effects and analysis of Phytoestrogene contents (see Table 4).

Table 4. Standards for the Detection of Phytoestrogens

Catalog No. Product Name CAS No. Description Package Size
00017 Acacetin 480-44-4 BioChemika; >99.0% (TLC) 25 mg, 100 mg
10798 Apigenin 520-36-5 BioChemika; ~98% (HPLC) 25mg, 100 mg
14385 Biochanin A 491-80-5 BioChemika; >97.0% (TLC) 100 mg, 1g
27214 Chrysin 480-40-0 BioChemika; ~98% (TLC) 100 mg, 1g
30405 Daidzein 486-66-8 purum; >98.0% (TLC) 5mg, 25 mg
30408 Daidzin 552-66-9 BioChemika; ~97% (HPLC) 1mg, 5mg
38763 4',7-Dimethoxyisoflavone 1157-39-7 BioChemika; ~98% (TLC) 1mg, 5mg
45405 Equol 94105-90-5 BioChemika; >99.0% (TLC ) 1mg, 5mg
46340 Fisetin 528-48-3 BioChemika; >99.0% (TLC) 5 mg, 100 mg
48754 Genistein 4',7-dimethyl ether 34086-51-6 BioChemika; ~95% (TLC) 1mg, 5mg
48756 Genistin 529-59-9 BioChemika; >99.0% (TLC) 1mg, 5mg
60010 Kaempferol 520-18-3 BioChemika; >96% (HPLC) 25mg, 100 mg
75375 Ononin 486-62-4 BioChemika; >99.0% (TLC) 1mg, 5mg
80614 Pinostrobin 480-37-5 BioChemika; >99.0% (TLC) 5mg, 25 mg
82415 Prunetin 552-59-0 BioChemika; >99.0% (TLC) 5mg, 25 mg
82435 Puerarin 3681-99-0 BioChemika; >99.0% (TLC) 1mg, 5mg
83370 Quercetin Dihydrate 6151-25-3 BioChemika; ~99% (HPLC) 25g, 100 g
85447 Sissostrin 5928-26-7 BioChemika; ~95% (TLC) 1 mg
91953 3',4',7-Trihydroxyisoflavone 485-63-2 BioChemika; >99.0% (TLC) 1mg, 5mg
91955 4',5,7-Trihydroxyisoflavone 485-63-2 BioChemika; >98.0% (HPLC) 25mg, 100 mg
91956 4',6,7-Trihydroxyisoflavone 17817-31-1 BioChemika; ~98% (TLC) 1mg, 5mg
92182 4',6,7-Trimethoxyisoflavone 798-61-8 BioChemika; >99.0% (TLC) 1mg, 5mg
96093 Zearalenone 17924-92-4 BioChemika; >98.0% (TLC) 5mg, 25 mg


GMO Certified Reference Material from IRMM

The Certified Reference Materials (CRMs) for detection of material derived from genetically modified plants (GMOs) were developed and produced by IRMM (Inst. for Reference Materials and Measurement, Geel, Belgium) on behalf of Fluka GmbH (Buchs, Switzerland) in the frame of a collaboration with the Environment Institute (EI) of the Joint Research Centre of the European Comission (Ispra, Italy). They consist of dried flour containing different mass fractions of powder from genetically modified soya beans or maize kernels respectively.


New Generation of CRMs for Roundup Ready™ Soya

Five CRMs of dried soya bean powder with different mass fractions (0 nominal (< 0.1), 0.5, 1, 2 and 5 %) of genetically modified "Roundup Ready™" soya are available now. These CRMs, IRMM-410S, are replacing IRMM-410R, which was sold out in January 2002. In contrary to IRMM-410R, IRMM-410S has been produced with the help of dry-mixing techniques (3rd generation) in order to minimise DNA and protein degradation occurring during the production. The reference material has been produced from whole seeds of a non-modified soya line (Asgrow line A1900) and genetically modified event 40-3-2 Roundup Ready™ soya (Asgrow line AG5602 RR), both delivered by Monsanto, St.Louis, MO, USA.

The non-GMO raw material used for the production con-tains traces of Roundup Ready™ soya GMO below 0.03 %. IRMM-410S is intended to be used for the validation of methods for the detection of genetically modified food and is available in the form of glass bottles containing 1 g of soya bean powder packed under argon atmosphere.

Table 5. Roundup Ready™ Soya Standards

Catalog No. Product Name Description Package Size
83063 Soya Bean Powder, Cert. Ref. Material IRMM-410S-0 Replacement for 53198
0 % (nominal, < 0.03%) genetically modified RRTM soya
1 g
72647 Soya Bean Powder, Cert. Ref. Material IRMM-410S-1 Replacement for 49232
0.1 % genetically modified RRTM soya
1 g
81751 Soya Bean Powder, Cert. Ref. Material IRMM-410S-2 Replacement for 57452
0.5 % genetically modified RRTM soya
1 g
76913 Soya Bean Powder, Cert. Ref. Material IRMM-410S-3 Replacement for 68202
1 % genetically modified RRTM soya
1 g
78767 Soya Bean Powder, Cert. Ref. Material IRMM-410S-4 Replacement for 49215
2 % genetically modified RRTM soya
1 g
93109 Soya Bean Powder, Cert. Ref. Material IRMM-410S-5 Replacement for 44386
5 % genetically modified RRTM soya
1 g
94162 Soya Bean Powder set, Cert. Ref. Material IRMM-410S Replacement for 89305
0, 0.1, 0.5, 1, 2 and 5 % genetically modified RRTM soya
1 set


Table 6. Maize MON 810 Standards


Catalog No. Product Name Description Package Size
77119 IRMM-413-0 for 0% MON 810 0% (nominal) genetically modified MON 810 maize 1 g
74411 IRMM-413-1 for 0.1% MON 810 0.1% genetically modified MON 810 maize 1 g
79521 IRMM-413-2 for 0.5% MON 810 0.5% genetically modified MON 810 maize 1 g
71981 IRMM-413-3 for 1% MON 810 1% genetically modified MON 810 maize 1 g
79112 IRMM-413-4 for 2% MON 810 2% genetically modified MON 810 maize 1 g
76182 IRMM-413-5 for 5% MON 810 5% genetically modified MON 810 maize 1 g
78761 IRMM-413-Set for 0%, 0.1%, 0.5%, 1%, 2% and 5% MON 810 - set


Table 7. IRMM Certified Standards for Maize Bt-11 and Maize Bt-176

Catalog No. Product Package Size
Maize Bt-176
03828 Maize GMO Standard IRMM 411R, Set for 0%, 0.1%, 0.5%, 1%, 2%, 5% Bt-176 1 set
91528 Maize GMO Standard IRMM 411R-0, 0% Bt-176 1 g
91516 Maize GMO Standard IRMM 411R-1, 0.1% Bt-176 1 g
14724 Maize GMO Standard IRMM 411R-2, 0.5% Bt-176 1 g
95772 Maize GMO Standard IRMM 411R-3, 1% Bt-176 1 g
89092 Maize GMO Standard IRMM 411R-4, 2% Bt-176 1 g
19074 Maize GMO Standard IRMM 411R-5, 5% Bt-176 1 g
Maize Bt-11
03831 Maize GMO Standard IRMM 412R, Set for 0%, 0.1%, 0.5%, 1%, 2%, 5% Bt-11 1 set
91524 Maize GMO Standard IRMM 412R-0, 0% Bt-11 1 g
54703 Maize GMO Standard IRMM 412R-1, 0.1% Bt-11 1 g
52492 Maize GMO Standard IRMM 412R-2, 0.5% Bt-11 1 g
19577 Maize GMO Standard IRMM 412R-3, 1% Bt-11 1 g
19558 Maize GMO Standard IRMM 412R-4, 2% Bt-11 1 g
65944 Maize GMO Standard IRMM 412R-5, 5% Bt-11 1 g


GMO Qualitative Standards

In April 2004 the legislation regarding the labelling of genetically modified foods and feed products changed. This new legislation requires that even highly modified foods such as lecithin, starch and oil, as well as feed products containing GMOs are labelled clearly as such.Therefore, maize and canola are now subject to the new labelling legislation, requiring more stringent control and labelling than was previously required.

However, in order to measure the presence or absence of GMOs a suitable standard is required. Fluka is now presenting a new range of GMO Maize Standards (NK603-, GA21- and CBH-3511 Starlink varieties) and Canola (RT73-, OXY235-, LibertyLink Falcon®- or MS8xRf3- varieties). These will enable you to easily identify manipulated material

There are numerous benefits to be gained from the use of Fluka GMO standards:

  • The process of DNA extraction for use as a positive control can be avoided, thereby eliminating the need for time-consuming manual extraction procedures.
  • Since the DNA is extracted from plant material, the application of these standards is no longer limited to a certain sequence information or protocol. Therefore, it is possible to use them with all DNA–based methods and even with your own optimized protocols
  • The world wide availability of GMO standards having the same characteristics and composition can ensure the standardization of measurements and procedures, therefore allowing comparison of results obtained in different facilities.


Table 8. GMO Qualitative Standards

Catalog No. Product  
55231 Canola GMO Genomic DNA Standard PDF
69407 Maiz GMO Genomic DNA Standard PDF

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