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Kits and Reagents for Food Analysis
Ready-to-use Kit for Acrylamide Analysis
Last year the Swedish National Food Administration published its concerns regarding the high concentrations of acrylamide detected in foods processed at high temperatures. This discovery was particularly disturbing since the acrylamide-containing food stuff included products that are consumed on a regular basis at rather large quantities, such as potato chips, French fries, roast potatoes, breakfast cereals and crisp bread.
The most common method for the determination of acrylamide in food is the Grob-Method. This method requires several high quality reagents and standards that are not available from the same supplier. Fluka has combined efforts and is now presenting a ready-to-use kit that makes acrylamide determination faster and easier. The Acrylamide Kit (Cat. No 72615) contains all the necessary standards and solvents for the extraction and accurate quantification of acrylamide as proposed by Grob1.
Your convenient solution: Acrylamide Kit
It was an exciting challenge for us to provide you with a ready-to-use kit for fast and easy determination of acrylamide. The Kit (Cat. No 72615) contains all reagents and standards needed for 12 determinations. The suitability of all 8 reagents was thoroughly tested. The concentrations and specifications of the Acrylamide-, Acrylamide-D3- and Methacrylamide-Standard Solutions were specially tailored for this method which reduces the time required significantly. The kit contains all the instructions required for sample preparation. Table 1 provides an overview of the kit and the reagents, their specifications and pack sizes. Please click here to download the method (168 KB).
Composition of Acrylamide Kit (Cat. No 72615)
| Component |
Specification |
Pack Size |
| Acrylamide Standard Solution |
500 ppm in Acetonitrile |
5 ml (Certan® vial) |
| Acrylamide-D3 Standard Solution |
500 ppm in Acetonitrile |
5 ml (Certan® vial) |
| Methylacrylamide Standard Solution |
500 ppm in Acetonitrile |
5 ml (Certan® vial) |
| Butyramide Standard Solution |
25 ppm in Acetonitrile |
5 ml (Certan® vial) |
| 1-Propanol |
puriss.p.a., >99.5 % (GC) |
250 ml glass bottle |
| n-Hexane |
puriss., >99.0 % (GC) |
100 ml glass bottle |
| Acetonitrile |
for residue analysis, >99.9 % (GC) |
50 ml glass bottle |
| Oil |
tested to determine Acrylamide
according to Grob |
50 ml glass bottle |
Table 1: Components of Acrylamide Kit (Cat. No 72615). Standard solutions are available as kit only and can not be ordered individually. All components last for 12 determinations.
Literature References
1 M. Biedermann, S. Biedermann-Brem, A. Noti and K. Grob; P. Egli and H. Mändli; Mitt. Lebensm. Hyg. 2002, 93, 638-652
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Standards for food analysis: BADGE, BFDGE
Epoxy based resins are widely used for lacquer coatings on the inside of food cans and food storage vessels. BADGE and/or BFDGE are normally added to these coatings. Studies have shown that the hydrochloric media in the edible oil reacts with the epoxy based resins of the coating, resulting in the corresponding HCl-adducts, of which its toxicities are unknown. BADGE and/or BFDGE are added to the coating in order to prevent these reactions by reacting with hydrochloric acid itself.
There has been performed considerable research in Europe to develop and apply methods of analysis for the food analysis standards. In 2000 the European Scientific Committee for Food produced a proposal for a limit of 1 mg/kg for BADGE, BFDGE (and their adducts) as well as hydrolysates in canned food. For performing a reliable analysis, standards of BADGE, BFDGE and its derivatives are needed. Fluka offers 9 unique, carefully synthesized standards including: BADGE, BFDGE, the HCl-adducts and hydrolysates (see Table 1). There is a large target group of people working with these standards, including all analytical chemists within the food industry (control laboratories), can producing companies, governmental laboratories, private analytical service laboratories and university laboratories.
Table 1. Standards for Food Analysis: BADGE, BFDGE
| Catalog No. |
Product Name |
CAS No. |
Description |
Package Size |
| 15136 |
Bisphenol A bis(3-chloro-2-hydroxypropyl) ether |
4809-35-2 |
purum p.a. qualitative standard; >99.0% (HPLC) |
250 mg, 1g |
| 15137 |
Bisphenol A bis(2,3-dihydroxypropyl)ether |
5581-32-8 |
purum p.a. qualitative standard; >97.0% (HPLC) |
250 mg |
| 92427 |
Bisphenol A (3-chloro-2-hydroxypropyl) (2,3-dihydroxypropyl) ether |
227947-06-0 |
purum p.a. qualitative standard; >98.0% (HPLC) |
25 mg, 100 mg |
| 73124 |
Bisphenol A (3-chloro-2-hydroxypropyl) glycidyl ether |
13836-48-1 |
purum p.a. qualitative standard; ~95% (HPLC) |
25 mg, 100 mg |
| 15138 |
Bisphenol A diglycidyl ether, standardfor quantification |
1675-54-3 |
purum p.a. qualitative standard; >97.0% (GC) |
500 mg |
| 73417 |
Bisphenol A (2,3-dihydroxypropyl) glycidyl ether |
76002-91-0 |
purum p.a. qualitative standard; ~97% (HPLC) |
25 mg, 100 mg |
| 15139 |
Bisphenol F bis(3-chloro-2-hydroxy-propyl) ether |
- |
p.a. qualitative standard; mixture of 3 isomers ortho-ortho, ortho-para, para-para; ~95% (HPLC) total assay of the 3 isomers |
250 mg |
| 15142 |
Bisphenol F bis(2,3-dihydroxypropyl)ether |
72406-26-9 |
purum p.a. qualitative standard; mixture of 3 isomers ortho-ortho, ortho-para, para-para; >97.0% (HPLC) total assay of the 3 isomers |
250 mg |
| 15144 |
Bisphenol F diglycidyl ether |
2095-03-6 |
purum p.a. qualitative standard; mixture of 3 isomers ortho-ortho, ortho-para, para-para; ~97% (GC) total assay of the 3 isomers |
500 mg |
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Food Irradiation
Treatment of food-stuff with ionized radiation is used to eliminate microbiological activity. Such food-stuff, with a few exceptions, cannot be sold in certain European countries and need to be declared in many other countries. Irradiation produces certain substances which are absent in untreated food stuff and have been used as indicators of irradiation. Irradiation of fat-containing foodstuff (e.g. chicken meat, hazelnut) produces free radicals, which partially degrade fat (triglycerides). This process produces alkanes, alkenes, aldehydes and alkylcyclobutanones. Irradiation of water-containing albuminous foodstuffs (meat) produces reactive hydroxyradicals, which react with phenylalanine to produce ortho- and meta-tyrosine. Detection of typical radiation byproducts is used to prove treatment with ionized radiation. Fluka offers selected indicator substances which can be used as standards for GC-analysis (Table 2).
Table 2. Standards for the Detection of Food Irradiation
| Catalog No. |
Product Name |
CAS No. |
Description |
Package Size |
| 52206 |
1,7-Hexadecadiene |
125110-62-5 |
~ 95% (GC), standard for food analysis |
5 mg, 25 mg |
| 87141 |
1,7-Tetradecadiene |
150767-46-7 |
~ 95% (GC), standard for food analysis |
5 mg, 25 mg |
| 44197 |
2-Dodecylcyclobutanone |
35493-46-0 |
> 95% (GC), standard for food analysis |
5 mg, 25 mg |
| 93851 |
DL-o-Tyrosine |
2370-61-8 |
~ 98% (NT), standard for food analysis |
100 mg |
| 93853 |
DL-m-Tyrosine |
775-06-4 |
> 98.0% (NT), standard for food analysis |
100 mg |
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Phytoestrogens
It is well established, that plants contain substances with estrogenic effects, so called phytoestrogens. These substances have been found in numerous plant food products, e.g. from carrots, peas, lentil, rice, cabbage and soy. They are structurally similar to the mammalian estrogen or estradiol, and show estrogenic properties. However, their estrogenic activity is generally much less than that of human estrogens (estrogenic activity ranges from 1/500 to 1/1000 of the activity of oestradiol). Hence phytoestrogens can act as anti-oestrogenic agents by blocking the oestrogen receptors and exerting a much weaker oestrogenic effect compared with the hormone. As a consequence it has been suggested that they might partly suppress or inhibit normal oestrogenic activity in oestrogen-responsive tissues such as breast tissue and may reduce the risk of breast cancer. Fluka offers a large number of selected pure flavones, flavonones and isoflavones for the examination of phytoestrogenic effects and analysis of Phytoestrogene contents (see Table 3)
Table 3. Standards for the Detection of Phytoestrogens
| Catalog No. |
Product Name |
CAS No. |
Description |
Package Size |
| 00017 |
Acacetin |
480-44-4 |
BioChemika; >99.0% (TLC) |
25 mg, 100 mg |
| 10798 |
Apigenin |
520-36-5 |
BioChemika; ~98% (HPLC) |
25mg, 100 mg |
| 14385 |
Biochanin A |
491-80-5 |
BioChemika; >97.0% (TLC) |
100 mg, 1g |
| 27214 |
Chrysin |
480-40-0 |
BioChemika; ~98% (TLC) |
100 mg, 1g |
| 30405 |
Daidzein |
486-66-8 |
purum; >98.0% (TLC) |
5mg, 25 mg |
| 30408 |
Daidzin |
552-66-9 |
BioChemika; ~97% (HPLC) |
1mg, 5mg |
| 38763 |
4',7-Dimethoxyisoflavone |
1157-39-7 |
BioChemika; ~98% (TLC) |
1mg, 5mg |
| 45405 |
Equol |
94105-90-5 |
BioChemika; >99.0% (TLC ) |
1mg, 5mg |
| 46340 |
Fisetin |
528-48-3 |
BioChemika; >99.0% (TLC) |
5 mg, 100 mg |
| 48754 |
Genistein 4',7-dimethyl ether |
34086-51-6 |
BioChemika; ~95% (TLC) |
1mg, 5mg |
| 48756 |
Genistin |
529-59-9 |
BioChemika; >99.0% (TLC) |
1mg, 5mg |
| 60010 |
Kaempferol |
520-18-3 |
BioChemika; >96% (HPLC) |
25mg, 100 mg |
| 75375 |
Ononin |
486-62-4 |
BioChemika; >99.0% (TLC) |
1mg, 5mg |
| 80614 |
Pinostrobin |
480-37-5 |
BioChemika; >99.0% (TLC) |
5mg, 25 mg |
| 82415 |
Prunetin |
552-59-0 |
BioChemika; >99.0% (TLC) |
5mg, 25 mg |
| 82435 |
Puerarin |
3681-99-0 |
BioChemika; >99.0% (TLC) |
1mg, 5mg |
| 83370 |
Quercetin Dihydrate |
6151-25-3 |
BioChemika; ~99% (HPLC) |
25g, 100 g |
| 85447 |
Sissostrin |
5928-26-7 |
BioChemika; ~95% (TLC) |
1 mg |
| 91953 |
3',4',7-Trihydroxyisoflavone |
485-63-2 |
BioChemika; >99.0% (TLC) |
1mg, 5mg |
| 91955 |
4',5,7-Trihydroxyisoflavone |
485-63-2 |
BioChemika; >98.0% (HPLC) |
25mg, 100 mg |
| 91956 |
4',6,7-Trihydroxyisoflavone |
17817-31-1 |
BioChemika; ~98% (TLC) |
1mg, 5mg |
| 92182 |
4',6,7-Trimethoxyisoflavone |
798-61-8 |
BioChemika; >99.0% (TLC) |
1mg, 5mg |
| 96093 |
Zearalenone |
17924-92-4 |
BioChemika; >98.0% (TLC) |
5mg, 25 mg |
GMO Certified Reference Material from IRMM
The Certified Reference Materials (CRMs) for detection of material derived from genetically modified plants (GMOs) were developed and produced by IRMM (Inst. for Reference Materials and Measurement, Geel, Belgium) on behalf of Fluka GmbH (Buchs, Switzerland) in the frame of a collaboration with the Environment Institute (EI) of the Joint Research Centre of the European Comission (Ispra, Italy). They consist of dried flour containing different mass fractions of powder from genetically modified soya beans or maize kernels respectively.
New Generation of CRMs for Roundup ReadyTM Soya:
Five CRMs of dried soya bean powder with different mass fractions (0 nominal (< 0.1), 0.5, 1, 2 and 5 %) of genetically modified "Roundup ReadyTM" soya are available now. These CRMs, IRMM-410S, are replacing IRMM-410R, which was sold out in January 2002. In contrary to IRMM-410R, IRMM-410S has been produced with the help of dry-mixing techniques (3rd generation) in order to minimise DNA and protein degradation occurring during the production. The reference material has been produced from whole seeds of a non-modified soya line (Asgrow line A1900) and genetically modified event 40-3-2 Roundup ReadyTM soya (Asgrow line AG5602 RR), both delivered by Monsanto, St.Louis, MO, USA.
The non-GMO raw material used for the production con-tains traces of Roundup ReadyTM soya GMO below 0.03 %. IRMM-410S is intended to be used for the validation of methods for the detection of genetically modified food and is available in the form of glass bottles containing 1 g of soya bean powder packed under argon atmosphere.
Table 4. Roundup ReadyTM Soya Standards
| Catalog No. |
Product Name |
Description |
Package Size |
| 83063 |
Soya Bean Powder, Cert. Ref. Material IRMM-410S-0 |
Replacement for 53198
0 % (nominal, < 0.03%) genetically modified RRTM soya |
1 g |
| 72647 |
Soya Bean Powder, Cert. Ref. Material IRMM-410S-1 |
Replacement for 49232
0.1 % genetically modified RRTM soya |
1 g |
| 81751 |
Soya Bean Powder, Cert. Ref. Material IRMM-410S-2 |
Replacement for 57452
0.5 % genetically modified RRTM soya |
1 g |
| 76913 |
Soya Bean Powder, Cert. Ref. Material IRMM-410S-3 |
Replacement for 68202
1 % genetically modified RRTM soya |
1 g |
| 78767 |
Soya Bean Powder, Cert. Ref. Material IRMM-410S-4 |
Replacement for 49215
2 % genetically modified RRTM soya |
1 g |
| 93109 |
Soya Bean Powder, Cert. Ref. Material IRMM-410S-5 |
Replacement for 44386
5 % genetically modified RRTM soya |
1 g |
| 94162 |
Soya Bean Powder set, Cert. Ref. Material IRMM-410S |
Replacement for 89305
0, 0.1, 0.5, 1, 2 and 5 % genetically modified RRTM soya |
1 set |
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Table 5. Maize MON 810 Standards
| Catalog No. |
Product Name |
Description |
Package Size |
| 77119 |
IRMM-413-0 for 0% MON 810 |
0% (nominal) genetically modified MON 810 maize |
1 g |
| 74411 |
IRMM-413-1 for 0.1% MON 810 |
0.1% genetically modified MON 810 maize |
1 g |
| 79521 |
IRMM-413-2 for 0.5% MON 810 |
0.5% genetically modified MON 810 maize |
1 g |
| 71981 |
IRMM-413-3 for 1% MON 810 |
1% genetically modified MON 810 maize |
1 g |
| 79112 |
IRMM-413-4 for 2% MON 810 |
2% genetically modified MON 810 maize |
1 g |
| 76182 |
IRMM-413-5 for 5% MON 810 |
5% genetically modified MON 810 maize |
1 g |
| 78761 |
IRMM-413-Set for 0%, 0.1%, 0.5%, 1%, 2% and 5% MON 810 |
- |
set |
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Table 6. IRMM Certified Standards for Maize Bt-11 and Maize Bt-176
| Catalog No. |
Product |
Pack Size |
| Maize Bt-176 |
| 03828 |
Maize GMO Standard IRMM 411R, Set for 0%, 0.1%, 0.5%, 1%, 2%, 5% Bt-176 |
1 set |
| 91528 |
Maize GMO Standard IRMM 411R-0, 0% Bt-176 |
1 g |
| 91516 |
Maize GMO Standard IRMM 411R-1, 0.1% Bt-176 |
1 g |
| 14724 |
Maize GMO Standard IRMM 411R-2, 0.5% Bt-176 |
1 g |
| 95772 |
Maize GMO Standard IRMM 411R-3, 1% Bt-176 |
1 g |
| 89092 |
Maize GMO Standard IRMM 411R-4, 2% Bt-176 |
1 g |
| 19074 |
Maize GMO Standard IRMM 411R-5, 5% Bt-176 |
1 g |
| Maize Bt-11 |
| 03831 |
Maize GMO Standard IRMM 412R, Set for 0%, 0.1%, 0.5%, 1%, 2%, 5% Bt-11 |
1 set |
| 91524 |
Maize GMO Standard IRMM 412R-0, 0% Bt-11 |
1 g |
| 54703 |
Maize GMO Standard IRMM 412R-1, 0.1% Bt-11 |
1 g |
| 52492 |
Maize GMO Standard IRMM 412R-2, 0.5% Bt-11 |
1 g |
| 19577 |
Maize GMO Standard IRMM 412R-3, 1% Bt-11 |
1 g |
| 19558 |
Maize GMO Standard IRMM 412R-4, 2% Bt-11 |
1 g |
| 65944 |
Maize GMO Standard IRMM 412R-5, 5% Bt-11 |
1 g |
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GMO Qualitative Standards
In April 2004 the legislation regarding the labelling of genetically modified foods and feed products changed. This new legislation requires that even highly modified foods such as lecithin, starch and oil, as well as feed products containing GMOs are labelled clearly as such.Therefore, maize and canola are now subject to the new labelling legislation, requiring more stringent control and labelling than was previously required.
However, in order to measure the presence or absence of GMOs a suitable standard is required. Fluka is now presenting a new range of GMO Maize Standards (NK603-, GA21- and CBH-3511 Starlink varieties) and Canola (RT73-, OXY235-, LibertyLink Falcon®- or MS8xRf3- varieties). These will enable you to easily identify manipulated material
There are numerous benefits to be gained from the use of Fluka GMO standards:
| - |
The process of DNA extraction for use as a positive control can be avoided, thereby eliminating the need for time-consuming manual extraction procedures. |
| - |
Since the DNA is extracted from plant material, the application of these standards is no longer limited to a certain sequence information or protocol. Therefore, it is possible to use them with all DNA–based methods and even with your own optimized protocols |
| - |
The world wide availability of GMO standards having the same characteristics and composition can ensure the standardization of measurements and procedures, therefore allowing comparison of results obtained in different facilities. |
Table 7. GMO Qualitative Standards
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