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Determination of Free and Total Glycerin in B-100 Biodiesel by ASTM D6584 and EN 14105
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Biodiesel fuel must conform to ASTM D6751 specifications for use in diesel engines. Testing for this confirmation is outlined in ASTM D6584 and similar method DIN EN 14105. These methods provide for the quantitative determination of free and total glycerin in 100% biodiesel fuel (B100 methyl esters) by high temperature gas chromatography after silylating the sample with N-methyl-N-(trimethylsilyl) trifluoroacetamide (MSTFA).
Biodiesel Calibration Standards
Derivatization Reagents
Accessories – Vials and Syringes
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Supelco glycerin impurities in biodiesel reference solutions meet the specifications called out in ASTM D6584 and DIN EN 14105. Our reference solutions have been carefully formulated to save 4–6 hours in prep time, minimize the possibility of human error, and eliminate the need to prepare pyridine dilutions. Our standards offer:
- raw materials tested for identity and purity
- stock solutions for preparing your own multi-component solutions
- multilevel, multi-component solutions ready for derivatization
- monoglyceride commercial mixture for EN14105
- internal standard solutions
- certificate of composition shipped with each standard
- instructions for derivatization with each kit
Our standards are manufactured under the Quality Management System which is registered under ISO 9001:2000.
Ordering Information for Calibration Standards and Standard Solutions
| Description |
Cat. No. |
| ASTM D6584 |
| Glycerin, 1 mL, 500 µg/mL in pyridine |
44892-U |
| Monoolein, 3 mL, 5000 µg/mL in pyridine |
44893-U |
| Diolein solution, 2 mL, 5000 µg/mL in pyridine |
44894-U |
| Triolein, 2 mL, 5000 µg/mL in pyridine |
44895-U |
| Butanetriol Internal Standard #1, 5 mL, 1000 µg/mL in pyridine |
44896-U |
| Tricaprin Internal Standard #2, 5 mL, 8000 µg/mL in pyridine |
44897-U |
| ASTM D6584 Individual Stock and Internal Standards Mix Kit |
44898-U |
| ASTM D6584 Standard Solution 1, 1 mL, in pyridine (varied) |
44899-U |
| ASTM D6584 Standard Solution 2, 1 mL, in pyridine (varied) |
44914-U |
| ASTM D6584 Standard Solution 3, 1 mL, in pyridine (varied) |
44915-U |
| ASTM D6584 Standard Solution 4, 1 mL, in pyridine (varied) |
44916-U |
| ASTM D6584 Standard Solution 5, 1 mL, in pyridine (varied) |
44917-U |
| ASTM D6584 Individual Standard Solution & Internal Standards Kit |
44918-U |
| DIN EN 14105 |
| EN 14105:2003 Monoglyceride Stock Solution, 1 mL, 10 mg/mL in pyridine |
49446-U |
| EN 14105:2003 Standard Solution 1, 1 mL, in pyridine (varied) |
49441-U |
| EN 14105:2003 Standard Solution 2, 1 mL, in pyridine (varied) |
49442-U |
| EN 14105:2003 Standard Solution 3, 1 mL, in pyridine (varied) |
49443-U |
| EN 14105:2003 Standard Solution 4, 1 mL, in pyridine (varied) |
49444-U |
| EN 14105:2003 Standard Solution Kit |
49445-U |
MSTFA (N-methyl-N-(trimethylsilyl)trifluoroacetamide) is cited in DIN EN 14105 and ASTM D6584 for silylating the free and total glycerides of B-100 biodiesel prior to analysis by gas chromatography. MSTFA transforms the glycerides into a thermally stabile, but volatile, derivative for measuring glycerin impurities in biodiesel.
MSTFA is sensitive to moisture. We recommend purchasing the smallest quantity possible for your application, and storing the material in a desiccator at a temperature between 4-8 ºC
Ordering Information for MSTFA reagent
| Description |
Cat. No. |
| N-Methyl-N-(trimethylsilyl)trifluoroacetamide (derivatization grade) |
394866 |
Supelco offers a large selection of vials and syringes for most applications. Listed below are the vials and syringes recommended for use with Supelco brand biodiesel standards when following ASTM D6584 methodology.
Ordering Information for Vials
| Description |
Cat. No. |
| Vials, screw top with phenolic hole cap, pre-assembled, 15 mL, amber glass |
27014-U |
| Vials, screw top with phenolic hole cap, pre-assembled, 15 mL, clear glass |
27158 |
| Vials, screw top, amber glass (cap not included), 15 mL, amber glass, thread 18-400, O.D. × height 21 mm × 70 mm |
27088-U |
| Vials, screw top, graduated (vial only), 15 mL, clear glass (with graduations), thread 18-400, O.D. × height 21 mm × 70 mm |
27026 |
| Screw cap with hole, for use with 15 mL vial (with 18 mm thread), black phenolic cap |
27165 |
| Septa, PTFE/red rubber, for use with 15 mL vial, PTFE/red rubber, diam. × thickness 16 mm × 0.060 in. |
27167 |
Ordering Information for Syringes
| Description |
Cat. No. |
Hamilton® syringe, cemented needle, pt.#5
volume 100 µL, needle size 22s ga (cone tip, side-port) |
24535 |
Hamilton® syringe, 700 series, cemented needle
volume 100 µL, needle size 22s ga (bevel tip) |
20737 |
Hamilton® syringe, cemented needle, pt.#5
volume 250 µL, needle size 22s ga (cone tip, side-port) |
24536 |
Hamilton® syringe, 700 series, cemented needle
volume 250 µL, needle size 22s ga (bevel tip) |
20738 |
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