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Chiral column screening, method optimization, and small-scale enantiomeric purification services are available from Supelco/Sigma-Aldrich.
Please contact Supelco/Sigma-Aldrich Technical Service (Phone: 814-359-3041) to discuss your chiral application request; you may have to fill out the sample submission form (PDF version, Word version) ahead of time.
HPLC Chiral column screening
HPLC chiral column screening protocol includes 3 mobile phase conditions run using 6 different chiral stationary phases. Positive separation is verified on a separate system. Enantiomers are identified as (+) and (-) using the Chiralyser optical rotation detection system.
View report example: Dorzolamide HPLC Chiral Column Screening Report (155kb pdf)
GC Chiral column screening
GC column screening involves exploration of 4 GC chiral phases. Samples that require derivatization are verified by GC-MS.
HPLC and GC Chiral method optimization
To establish the most efficient method, an optimization study can be conducted as an outcome of the initial column screening. Method optimization may vary, depending on the intended use of the method. Some typical uses include:
· Isolation/purification of enantiomers
· Resolution of metabolites
· Establishment of minimum detection limits
· LC-MS compatible methods for clinical, stability, or dissolution studies
Typical experiments in the optimization study include modifying buffer and pH, organic modifier type and strength, and column temperature.
Small-scale enantiomeric purification
When isolation of enantiomers is required, purification is required. The output of a small-scale purification project can be milligram to gram quantities, depending on the needs of the client. Typical enantiomeric purity is 98% and verification is determined by analytical methods established in the screening study.
Pricing, Turnaround time, and Deliverables
HPLC and GC chiral column screening is typically completed within 3 working days. A full report is supplied and a column is recommended upon completion of the project. A charge for the service is incurred whether or not a separation is identified. At that time, optimization studies can be started as desired by the client. Standard pricing has been set up for chiral screening. Optimization and purification are quoted on a project-by-project basis. Contact Technical Service to discuss your requirements.
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