HPLC and GC Chiral Column Screening
HPLC chiral column screening includes a range of chiral stationary phases and separation modes. Positive separation is verified on a separate system. Enantiomers are identified as (+) and (-) using the Chiralyser optical rotation detection system, where possible.
GC column screening involves exploration of different GC chiral phases. Samples that require derivatization are verified by GC-MS.
HPLC and GC Chiral Method Optimization and Loading Studies
To establish the most efficient method, an optimization study can be conducted as an outcome of the initial column screening. Method optimization may vary, depending on the intended use of the method. Some typical uses include:
- Isolation/purification of enantiomers
- Resolution of metabolites
- Establishment of minimum detection limits
- LC-MS compatible methods for clinical, stability, or dissolution studies
Typical experiments in the optimization study include modifying buffer and pH, organic modifier type and strength, and column temperature.
If you want to move to preparative chiral HPLC, we can help by performing loading studies of your analyte under the preferred or recommended mobile phase and CSP combination.
Small-scale Enantiomeric Purification
When isolation of enantiomers is required, purification is required. The output of a small-scale purification project can be milligram to gram quantities, depending on the needs of the client. Typical enantiomeric purity is 98% and verification is determined by analytical methods established in the screening study.
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